The solution molecular weight and shape of the bacterial exopolysaccharides amylovoran and stewartan

被引：13

作者：

Jumel, K ^{[1
]}

Geider, K ^{[1
]}

Harding, SE ^{[1
]}

机构：

[1] MAX PLANCK INST CELL BIOL,D-68526 LADENBURG,GERMANY

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 1997年 / 20卷 / 04期

关键词：

exopolysaccharide; fire blight; molecular weight determination;

D O I：

10.1016/S0141-8130(97)00023-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Amylovoran, the acidic exopolysaccharide (EPS) of Erwinia amylovora, and stewartan, the capsular EPS of E. stewartii, were characterized by analytical ultracentrifugation and by size exclusion chromatography connected to dual detection of light scattering and mass. The average molecular weights of amylovoran and stewartan were determined as 1.0 x 10(6) and 1.7 x 10(6) Da, with polydispersity values (M-w/M-n) of 1.5 and 1.4, respectively. Based on the sugar composition and their molecular weight, both exopolysaccharides consist of approximately 1000 repeating units per molecule, this suggests a similar mechanism for chain length determination during biosynthesis of EPS in both organisms. (C) 1997 Elsevier Science B.V.

引用

页码：251 / 258

页数：8

共 27 条

[1] EXTRACELLULAR POLYSACCHARIDE OF ERWINIA-AMYLOVORA - CORRELATION WITH VIRULENCE [J].