Memy I: a novel murine model for uterine leiomyoma using adenovirus-enhanced human fibroid explants in severe combined immune deficiency mice

被引:37
作者
Hassan, Memy H. [1 ,3 ]
Eyzaguirre, Eduardo [2 ]
Arafa, Hossam M. M. [3 ]
Hamada, Farid M. A. [3 ]
Salama, Salama A. [1 ]
Al-Hendy, Ayman [4 ]
机构
[1] Univ Texas Med Branch, Dept Obstet & Gynecol, Galveston, TX USA
[2] Univ Texas Med Branch, Dept Pathol, Galveston, TX USA
[3] Al Azhar Univ, Fac Pharm, Dept Pharmacol & Toxicol, Cairo, Egypt
[4] Meharry Med Coll, Dept Obstet & Gynecol, Nashville, TN 37208 USA
关键词
cyclooxygenase-2; leiomyoma models; severe combined immunodeficient mice; uterine leiomyoma; vascular endothelial growth factor-A;
D O I
10.1016/j.ajog.2008.02.010
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
OBJECTIVE: This study was undertaken to develop a representative murine model for human leiomyoma. STUDY DESIGN: Human fibroid tumor tissues were cut into small pieces and treated with medium alone, adenoviral-beta-galactosidase, adenoviral-vascular endothelial growth factor-A, adenoviral-cyclooxygenase-2, or both adenoviral-vascular endothelial growth factor-A and adenoviral-cyclooxygenase-2. Tissue pieces were inserted subcutaneously in the flank of each severe combined immunodeficient mouse. The developed lesion was measured twice per week. Xenograft tissues were harvested after 30 days and analyzed. RESULTS: Tissue pieces transfected with both adenoviral-cyclooxygenase-2 and adenoviral-vascular endothelial growth factor-A continued to grow up to 30 days postimplantation. The number of proliferating and apoptotic cells, as well as the expression of smooth muscle actin, desmin, vimentin, estrogen receptors, and progesterone receptors was similar between retrieved grafts from that group and the original patient tissue. Furthermore, hematoxylin and eosin and Masson's Trichrome stains confirmed this similarity. CONCLUSION: Human uterine leiomyoma xenografts, pretreated with both adenoviral-cyclooxygenase-2 and adenoviral-vascular endothelial growth factor-A and implanted subcutaneously in severe combined immunodeficient mice, represent a novel model for human uterine leiomyoma.
引用
收藏
页码:156.e1 / 156.e8
页数:8
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