HPLC/CE-ESI-TOF-MS methods for the characterization of polyphenols in almond-skin extracts

被引：28

作者：

Arraez-Roman, David

Fu, Shaoping ^{[2
]}

Sawalha, Saleh M. S.

Segura-Carretero, Antonio ^{[1
]}

Fernandez-Gutierrez, Alberto

机构：

[1] Univ Granada, Res Grp FQM 297, Dept Analyt Chem, Fac Sci, E-18071 Granada, Spain

[2] Dalian Polytech Univ, Inst Chem & Applicat Plant Resources, Sch Biol & Food Engn, Dalian, Peoples R China

来源：

ELECTROPHORESIS | 2010年 / 31卷 / 13期

关键词：

Almond skin; CE; ESI-TOF-MS; HPLC; Phenolic compounds; VIRGIN OLIVE OIL; CAPILLARY ELECTROMIGRATION METHODS; CHROMATOGRAPHY-MASS SPECTROMETRY; PHENOLIC-COMPOUNDS; FOOD ANALYSIS; FLAVONOIDS; QUANTIFICATION; CONSTITUENTS; AGLYCONS;

D O I：

10.1002/elps.200900679

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

We have developed two rapid methods using CE and HPLC coupled to ESI-TOF-MS and both these methods have been compared for the separation and characterization of antioxidant phenolic compounds in almond-skin extract. Under optimum CE-ESI-TOF-MS conditions we achieved the determination of nine compounds from the polar fraction in 35 min. Furthermore, by using the HPLC-ESI-TOF-MS method, a total of 23 compounds corresponding to phenolic acids and the flavonoid family were identified from almond skin in only 9 min. The sensitivity, together with mass accuracy and true isotopic pattern of TOF-MS, allowed the identification of a broad series of known phenolic compounds present in almond-skin extracts using HPLC and CE as separation techniques.

引用

收藏

页码：2289 / 2296

页数：8

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