The identification and characterization of four laccases from the plant pathogenic fungus Rhizoctonia solani

被引：6

作者：

Wahleithner, JA ^{[1
]}

Xu, F ^{[1
]}

Brown, KM ^{[1
]}

Brown, SH ^{[1
]}

Golightly, EJ ^{[1
]}

Halkier, T ^{[1
]}

Kauppinen, S ^{[1
]}

Pederson, A ^{[1
]}

Schneider, P ^{[1
]}

机构：

[1] NOVO NORDISK AS,DK-2880 BAGSVAERD,DENMARK

来源：

CURRENT GENETICS | 1996年 / 29卷 / 04期

关键词：

laccas; phenol-oxidas; Rhizoctonia solani; fungi;

D O I：

10.1007/s002940050061

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Four distinct laccase genes, lcc1, lcc2, lcc3 and lcc4, have been identified in the fungus Rhizoctonia solani. Both cDNA and genomic copies of these genes were isolated and characterized. Hybridization analyses indicate that each of the four laccase genes is present in a single copy in the genome. The R. solani laccases can be divided into two groups based on their protein size, intron/exon organization, and transcriptional regulation. Three of these enzymes have been expressed in the fungus Aspergillus oryzae. Two of the recombinant laccases, r-lcc1 and r-lcc4 as well as the native lcc4 enzyme were purified and characterized. The purified proteins are homodimeric, comprised of two subunits of approximately 66 kDa for lcc4 and 50-100 kDa for the recombinant lcc1 protein. These laccases have spectral properties that are consistent with other blue copper proteins. With syringaldazine as a substrate, lcc4 has optimal activity at pH 7, whereas lcc1 has optimal activity at pH 6.

引用

页码：395 / 403

页数：9

共 35 条

[1] SEQUENCE AND MOLECULAR-STRUCTURE OF THE ASPERGILLUS-NIDULANS YA (LACCASE-I) GENE [J].