Random amplified polymorphic DNA variation within and among bean landrace mixtures (Phaseolus vulgaris L.) from Tanzania

被引:14
作者
Briand, L
Brown, AE
Lenne, JM
Teverson, DM
机构
[1] Queens Univ Belfast, Dept Appl Plant Sci, Belfast BT9 5PX, Antrim, North Ireland
[2] Int Crops Res Inst Semi Arid Trop, Crop Protect Div, Patancheru, Andhra Pradesh, India
[3] Hort Res Int, Warwick CV35 9EF, England
[4] Nat Resources Inst, Chatham ME4 4TB, Kent, England
关键词
Phaseolus vulgaris; common bean; landraces; genetic diversity; RAPD analysis;
D O I
10.1023/A:1018365818566
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Genetic characterization of 51 individual pure lines from 13 landraces of three common bean (Phaseolus vulgaris L.) mixtures from the southern highlands of Tanzania was undertaken using random amplified polymorphic DNA (RAPD) analysis. A dendrogram generated by cluster analysis from data derived from fragments amplified by 12 random 10-base primers divided the bean individuals onto two main branches with less than 60% genetic similarity. Branches A and B subdivided into two and four clusters, respectively. Mixture 2, comprising three landraces, was the most uniform, most plants appearing on cluster 4 of branch B. Three of the four landraces of mixture 1 appeared on cluster 3 of branch B while the fourth landrace appeared on major branch A. Mixture 3 showed the greatest genetic variation with components appearing on both major branches. The clear separation of the 13 landraces onto two main branches of the dendrogram together with phenotypic characters, notably variation in bean size, suggests that the two groups might represent two distinct gene pools of P. vulgaris.
引用
收藏
页码:371 / 377
页数:7
相关论文
共 29 条
[1]  
ALLEN DJ, 1983, PATHOLOGY TROPICAL F
[2]   HARVEST SECURITY AND INTRASPECIFIC DIVERSITY IN TRADITIONAL TROPICAL AGRICULTURE [J].
CLAWSON, DL .
ECONOMIC BOTANY, 1985, 39 (01) :56-67
[3]  
DAVIS JHC, 1992, P 1 AFR BEAN PATH WO, P62
[4]   GENETIC DIVERSITY AND ECOLOGICAL DISTRIBUTION OF PHASEOLUS-VULGARIS (FABACEAE) IN NORTHWESTERN SOUTH-AMERICA [J].
DEBOUCK, DG ;
TORO, O ;
PAREDES, OM ;
JOHNSON, WC ;
GEPTS, P .
ECONOMIC BOTANY, 1993, 47 (04) :408-423
[5]  
Evans A. M., 1976, Evolution of crop plants., P168
[6]  
FELSENSTEIN J, 1993, PHYLIP 3 5 C
[7]   Genetic diversity in Lima bean (Phaseolus lunatus L) as revealed by RAPD markers [J].
Fofana, B ;
Vekemans, X ;
duJardin, P ;
Baudoin, JP .
EUPHYTICA, 1997, 95 (02) :157-165
[8]   Ecogeographic distribution of Phaseolus spp (Fabaceae) in Bolivia [J].
Freyre, R ;
Rios, R ;
Guzman, L ;
Debouck, DG ;
Gepts, P .
ECONOMIC BOTANY, 1996, 50 (02) :195-215
[9]  
GEPTS P, 1993, EVOL BIOL, V27, P51
[10]  
GEPTS P, 1986, ECON BOT, V40, P451, DOI 10.1007/BF02859660