Reconstitution of functional nuclear receptor proteins using high pressure refolding

被引：21

作者：

Schoner, BE

Bramlett, KS

Guo, HH

Burris, TP ^{[1
]}

机构：

[1] Lilly Corp Ctr, Lilly Res Labs, Indianapolis, IN 46285 USA

[2] Indiana Univ, Sch Med, Dept Pharmacol & Toxicol, Indianapolis, IN 46202 USA

来源：

MOLECULAR GENETICS AND METABOLISM | 2005年 / 85卷 / 04期

关键词：

steroid receptor; estrogen receptor; FXR; LRH-1; orphan receptor; nuclear receptor; bile acid; protein expression;

D O I：

10.1016/j.ymgme.2005.04.012

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Members of the nuclear receptor superfamily are ligand dependent transcription factors and many of the receptors are difficult to produce in their functional form. Here, we describe a method for obtaining functional nuclear receptor ligand binding domain proteins from bacterial expressed inclusion bodies by high hydrostatic pressure induced refolding. High pressure refolding successfully reconstituted activity from several insoluble nuclear receptor proteins and represents a valuable tool for both functional and structural investigation of proteins or fragments thereof that might otherwise remain insoluble. (c) 2005 Elsevier Inc. All rights reserved.

引用

收藏

页码：318 / 322

页数：5

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