Simultaneous saccharification and fermentation of microwave/alkali pre-treated rice straw to ethanol

The simultaneous saccharification and fermentation (SSF) of two different pre-treated rice straws to ethanol were optimised using cellulase from Trichoderma reesei and Saccharomyces cerevisiae YC-097 cells, respectively. The SSF optima for microwave/alkali pre-treated rice straw were 100 g l(-1) substrate, 40 degrees C, 15 mg [cellulase] g(-1) [substrate], initial pH 5(.)3 and 72h. Under the above optimum conditions ethanol concentration reached 25(.)8 g l(-1) and ethanol yield was 57(.)5%. The SSF optima for rice straw pre-treated by alkali alone were 100 g l(-1) substrate, 40 degrees C, 20 mg [cellulase] g(-1) [substrate], initial pH 5(.)3 and 96 h. Under these optimum conditions ethanol concentration and ethanol yield were 23(.)7 g l(-1) and 52(.)8%, respectively. It was obvious that production of ethanol from microwave/alkali pre-treated rice straw had lower enzyme loading, shorter reaction time, and could achieve higher ethanol concentration and yield than rice straw pre-treated by alkali alone. The microwave/alkali pre-treatment was an efficient pre-treatment method of rice straw for its ethanol production. (c) 2005 Silsoe Research Institute. All rights reserved Published by Elsevier Ltd.