Molecular diversity of 16S rRNA and gyrB genes in copper mines

被引:35
作者
Yin, Huaqun [1 ]
Cao, Linhui [1 ]
Qiu, Guanzhou [1 ]
Wang, Dianzuo [1 ]
Kellogg, Laurie [2 ,3 ]
Zhou, Jizhong [2 ,3 ]
Liu, Xinxing [1 ]
Dai, Zhimin [1 ]
Ding, Jiannan [1 ]
Liu, Xueduan [1 ]
机构
[1] Cent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Hunan, Peoples R China
[2] Univ Oklahoma, Stephenson Res & Technol Ctr, Inst Environm Genom, Norman, OK 73019 USA
[3] Univ Oklahoma, Stephenson Res & Technol Ctr, Dept Bot & Microbiol, Norman, OK 73019 USA
基金
中国国家自然科学基金;
关键词
molecular diversity; 16S rRNA and gyrB gene; geochemical characteristics; principal-component analysis;
D O I
10.1007/s00203-007-0298-6
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The molecular diversities of the microbial communities from four sites impacted by acid mine drainage (AMD) at Dexing Copper Mine in Jiangxi province of China were studied using 16S rRNA sequences and gyrB sequences. Of the four sampled sites, each habitat exhibited distinct geochemical characteristics and the sites were linked geographically allowing us to correlate microbial community structure to geochemical characteristics. In the present study, we examined the molecular diversity of 16S rRNA and gyrB genes from water at these sites using a PCR-based cloning approach. We found that the microbial community appears to be composed primarily of Proteobacteria, Acidobacteria, Actinobacteria, Nitrospira, Firmicutes, Chlorella and unknown phylotypes. Of clones affiliated with Nitrospira, Leptospirillum ferrooxidans, Leptospirillum ferriphilum and Leptospirillum group III were all detected. Principal-component analysis (PCA) revealed that the distribution of the microbial communities was influenced greatly by geochemical characteristics. The overall PCA profiles showed that the sites with similar geochemical characteristics had more similar microbial community structures. Moreover, our results also indicated that gyrB sequence analysis may be very useful for differentiating very closely related species in the study of microbial communities.
引用
收藏
页码:101 / 110
页数:10
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