Gene transfer mediated by alpha(2)-macroglobulin

被引：17

作者：

Schneider, H ^{[1
]}

Huse, K ^{[1
]}

Birkenmeier, G ^{[1
]}

Otto, A ^{[1
]}

Scholz, GH ^{[1
]}

机构：

[1] UNIV LEIPZIG,DEPT BIOCHEM,D-04103 LEIPZIG,GERMANY

来源：

NUCLEIC ACIDS RESEARCH | 1996年 / 24卷 / 19期

关键词：

D O I：

10.1093/nar/24.19.3873

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

alpha(2)-Macroglobulin covalently linked to poly(L)-lysine can be used as a vehicle for receptor-mediated gene transfer,This modified alpha(2)-macroglobulin maintains its ability to bind to the alpha(2)-macroglobulin receptor, and was shown to introduce a luciferase reporter gene plasmid into HepG2 human hepatoma cells in vitro. The alpha(2)-macroglobulin receptor is a very large and multifunctional cell surface receptor, whose rapid and efficient internalization rate makes it attractive for gene therapy, e.g. for hepatic gene targeting via injection into the portal vein.

引用

页码：3873 / 3874

页数：2

共 15 条

[1]

BIRKENMEIER G, 1994, METHOD ENZYMOL, V228, P264

[2] A VERSATILE METHOD FOR THE COUPLING OF PROTEIN TO DNA - SYNTHESIS OF ALPHA-2-MACROGLOBULIN-DNA CONJUGATES [J].