Genome analysis of a Glossina pallidipes salivary gland hypertrophy virus reveals a novel, large, double-stranded circular DNA virus

被引:65
作者
Abd-Alla, Adly M. M. [1 ,3 ]
Cousserans, Francois [2 ]
Parker, Andrew G. [1 ]
Jehle, Johannes A. [4 ]
Parker, Nicolas J.
Vlak, Just M. [5 ]
Robinson, Alan S. [1 ]
Bergoin, Max [2 ]
机构
[1] FAO, IAEA, Agri & Biotechnol Lab, IAEA Labs, A-2444 Seibersdorf, Austria
[2] Univ Montpellier 2, Lab Pathol Comparee, Montpellier, France
[3] Natl Res Ctr, Dept Pests & Plant Protect, Giza, Egypt
[4] Agr Serv Ctr Palatinate, DLR Rheinpfalz, D-67435 Neustadt, Germany
[5] Univ Wageningen & Res Ctr, Virol Lab, NL-6709 PD Wageningen, Netherlands
关键词
D O I
10.1128/JVI.02588-07
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Several species of tsetse flies can be infected by the Glossina pallidipes salivary gland hypertrophy virus (GpSGHV). Infection causes salivary gland hypertrophy and also significantly reduces the fecundity of the infected flies. To better understand the molecular basis underlying the pathogenesis of this unusual virus, we sequenced and analyzed its genome. The GpSGRV genome is a double-stranded circular DNA molecule of 190,032 bp containing 160 nonoverlapping open reading frames (ORFs), which are distributed equally on both strands with a gene density of one per 1.2 kb. It has a high A+T content of 72%. About 3% of the GpSGRV genome is composed of 15 sequence repeats, distributed throughout the genome. Although sharing the same morphological features (enveloped rod-shaped nucleocapsid) as baculoviruses, nudiviruses, and nimaviruses, analysis of its genome revealed that GpSGRV differs significantly from these viruses at the level of its genes. Sequence comparisons indicated that only 23% of GpSGHV genes displayed moderate homologies to genes from other invertebrate viruses, principally baculoviruses and entomopoxviruses. Most strikingly, the GpSGHV genome encodes homologues to the four baculoviral per os infectivity factors (p74 [pif-0], pif-1, pif-2, and pif-3). The DNA polymerase encoded by GpSGHV is of type B and appears to be phylogenetically distant from all DNA polymerases encoded by large double-stranded DNA viruses. The majority of the remaining ORFs could not be assigned by sequence comparison. Furthermore, no homologues to DNA-dependent RNA polymerase subunits were detected. Taken together, these data indicate that GpSGHV is the prototype member of a novel group of insect viruses.
引用
收藏
页码:4595 / 4611
页数:17
相关论文
共 85 条
[1]   Development of a non-destructive PCR method for detection of the salivary gland hypertrophy virus (SGHV) in tsetse flies [J].
Abd-Alla, Adly ;
Bossin, Herve ;
Cousserans, Francois ;
Parker, Andrew ;
Bergoin, Max ;
Robinson, Alan .
JOURNAL OF VIROLOGICAL METHODS, 2007, 139 (02) :143-149
[2]   The genome of Melanoplus sanguinipes Entomopoxvirus [J].
Afonso, CL ;
Tulman, ER ;
Lu, Z ;
Oma, E ;
Kutish, GF ;
Rock, DL .
JOURNAL OF VIROLOGY, 1999, 73 (01) :533-552
[3]   Interactions among multiple genomes: Tsetse, its symbionts and trypanosomes [J].
Aksoy, S ;
Rio, RVM .
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2005, 35 (07) :691-698
[4]   PRIMARY STRUCTURE OF THE HERPESVIRUS SAIMIRI GENOME [J].
ALBRECHT, JC ;
NICHOLAS, J ;
BILLER, D ;
CAMERON, KR ;
BIESINGER, B ;
NEWMAN, C ;
WITTMANN, S ;
CRAXTON, MA ;
COLEMAN, H ;
FLECKENSTEIN, B ;
HONESS, RW .
JOURNAL OF VIROLOGY, 1992, 66 (08) :5047-5058
[5]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[6]  
AMARGIER A, 1979, CR ACAD SCI D NAT, V289, P481
[7]   Sequence and organization of the Heliothis virescens ascovirus genome [J].
Asgari, Sassan ;
Davis, John ;
Wood, David ;
Wilson, Peter ;
McGrath, Annette .
JOURNAL OF GENERAL VIROLOGY, 2007, 88 :1120-1132
[8]   Complete genomic sequence of the Amsacta moorei entomopoxvirus:: Analysis and comparison with other poxviruses [J].
Bawden, AL ;
Glassberg, KJ ;
Diggans, J ;
Shaw, R ;
Farmerie, W ;
Moyer, RW .
VIROLOGY, 2000, 274 (01) :120-139
[9]   Tandem repeats finder: a program to analyze DNA sequences [J].
Benson, G .
NUCLEIC ACIDS RESEARCH, 1999, 27 (02) :573-580
[10]   Genomic sequence of Spodoptera frugiperda ascovirus 1a, an enveloped, double-stranded DNA insect virus that manipulates apoptosis for viral reproduction [J].
Bideshi, Dennis K. ;
Demattei, Marie-Veronique ;
Rouleux-Bonnin, Florence ;
Stasiak, Karine ;
Tan, Yeping ;
Bigot, Sylvie ;
Bigot, Yves ;
Federici, Brian A. .
JOURNAL OF VIROLOGY, 2006, 80 (23) :11791-11805