Inhibition of TNF-α-Induced Inflammation by Andrographolide via Down-Regulation of the PI3K/Akt Signaling Pathway

被引：88

作者：

Chen, Haw-Wen ^{[1
]}

Lin, Ai-Hsuan ^{[2
]}

Chu, Hsing-Chin ^{[2
]}

Li, Chien-Chun ^{[2
]}

Tsai, Chia-Wen ^{[1
]}

Chao, Che-Yi ^{[3
]}

Wang, Chau-Jong ^{[4
]}

Lii, Chong-Kuei ^{[1
]}

Liu, Kai-Li ^{[2
,5
]}

机构：

[1] China Med Univ, Dept Nutr, Taichung 404, Taiwan

[2] Chung Shan Med Univ, Dept Nutr, Taichung 402, Taiwan

[3] Asia Univ, Dept Hlth & Nutr Biotechnol, Taichung 413, Taiwan

[4] Chung Shan Med Univ, Inst Biochem & Biotechnol, Taichung 402, Taiwan

[5] Chung Shan Med Univ Hosp, Dept Dietitian, Taichung 402, Taiwan

来源：

JOURNAL OF NATURAL PRODUCTS | 2011年 / 74卷 / 11期

关键词：

NF-KAPPA-B; ADHESION MOLECULE-1 EXPRESSION; E-SELECTIN; ENDOTHELIAL-CELLS; NITRIC-OXIDE; ATHEROSCLEROSIS; ACTIVATION; MECHANISMS; ICAM-1; TRANSCRIPTION;

D O I：

10.1021/np200631v

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

Andrographolide (1), an active constituent of Andrographis paniculata, decreased tumor necrosis factor-alpha (TNF-alpha)-induced intercellular adhesion molecule-1 (ICAM-1) expression and adhesion of HL-60 cells onto human umbilical vein endothelial cells (HUVEC), which are associated with inflammatory diseases. Moreover, 1 abolished TNF-alpha-induced Akt phosphorylation. Transfection of an activated Akt1 cDNA vector increased Akt phosphorylation and ICAM-1 expression like TNF-alpha. In addition, 1 and LY294002 blocked TNF-alpha-induced IKB-alpha degradation and nuclear p65 protein accumulation, as well as the DNA-binding activity of NF-kappa 13. Compound 1 exhibits anti-inflammatory properties through the inhibition of TNF-alpha-induced ICAM-1 expression. The anti-inflammatory activity of 1 may be associated with the inhibition of the PI3K/Akt pathway and downstream target NF-kappa B activation in HUVEC cells.

引用

页码：2408 / 2413

页数：6

共 45 条

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