Synthesis, cloning and expression of genes for antibacterial peptides: Cecropin, magainin and bombinin.

被引：20

作者：

Martemyanov, KA ^{[1
]}

Spirin, AS ^{[1
]}

Gudkov, AT ^{[1
]}

机构：

[1] RUSSIAN ACAD SCI, INST PROT RES, PUSHCHINO 142292, RUSSIA

来源：

BIOTECHNOLOGY LETTERS | 1996年 / 18卷 / 12期

关键词：

D O I：

10.1007/BF00129335

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Three DNA sequences encoding the antimicrobial peptides bombinin, cecropin and magainin were synthesised. DNA fragments were cloned into pET-21d plasmid under T7 promoter for expression in vivo and in vitro and into pRIT-2T plasmid for expression as a fusion product with protein A. The polypeptides synthesised in both systems possess antibacterial activity.

引用

页码：1357 / 1362

页数：6

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