The human serum deprivation response gene (SDPR) maps to 2q32-q33 and codes for a phosphatidylserine-binding protein

被引:61
作者
Gustincich, S
Vatta, P
Goruppi, S
Wolf, M
Saccone, S
Della Valle, G
Baggiolini, M
Schneider, C
机构
[1] Lab Nazl Consorzio Interunit Biotechnol, CIB, I-34012 Trieste, Italy
[2] Univ Bern, Theodor Kocher Inst, CH-3012 Bern, Switzerland
[3] Univ Catania, Dipartimento Biol Anim, I-95124 Catania, Italy
[4] Univ Bologna, Dipartimento Biol, I-40126 Bologna, Italy
[5] Univ Udine, Dipartimento Sci & Tecnol Biomed, I-33100 Udine, Italy
关键词
D O I
10.1006/geno.1998.5733
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The serum deprivation response gene (SDPR, alias sdr) has been previously isolated for its high mRNA expression in serum-starved cells compared to contact-inhibited NIH3T3 cells; such regulation is not observed in single-oncogene transformed NIH3T3 cells after serum starvation. More recently Sdpr has been identified as a substrate of protein kinase C (PKC): this interaction determines the compartimentalization of PKC to caveolae, a plasma membrane invagination of which Sdpr is a major component. Lack of Sdpr-PKC interaction in transformed cells has been proposed to be involved in the alteration of PKC subcellular localization and substrate specificity, Here we report the cloning of the human SDPR homologue (HGMW-approved symbol SDPR) and its mapping to 2q32-q33 in the human genome. In analogy with the murine system, SDPR mRNA expression is increased when human fibroblasts are serum starved, it becomes down-regulated during synchronous cell-cycle reentry, but it is not induced in cells arrested by contact inhibition. Analysis of SDPR expression in human tissues reveals a near ubiquitous expression, with highest levels found in heart and lung. We show that human SDPR encodes PS-p68, a previously characterized phosphatidylserine-binding protein purified from human platelets. Accordingly, recombinant Sdpr is able to specifically bind phosphatidylserine in the absence of Ca2+. SDPR is homologous to two genes in the databank. one of which, srbc, is similarly regulated during growth arrest and encodes a phosphatidylserine-binding protein that is a substrate of PKC. (C) 1999 Academic Press.
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页码:120 / 129
页数:10
相关论文
共 49 条
  • [1] Phospholipid metabolism and membrane dynamics
    Alb, JG
    Kearns, MA
    Bankaitis, VA
    [J]. CURRENT OPINION IN CELL BIOLOGY, 1996, 8 (04) : 534 - 541
  • [2] COMPLEXITY OF THE EARLY GENETIC RESPONSE TO GROWTH-FACTORS IN MOUSE FIBROBLASTS
    ALMENDRAL, JM
    SOMMER, D
    MACDONALDBRAVO, H
    BURCKHARDT, J
    PERERA, J
    BRAVO, R
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (05) : 2140 - 2148
  • [3] BASIC LOCAL ALIGNMENT SEARCH TOOL
    ALTSCHUL, SF
    GISH, W
    MILLER, W
    MYERS, EW
    LIPMAN, DJ
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) : 403 - 410
  • [4] GAS2, A GROWTH ARREST SPECIFIC PROTEIN, IS A COMPONENT OF THE MICROFILAMENT NETWORK SYSTEM
    BRANCOLINI, C
    BOTTEGA, S
    SCHNEIDER, C
    [J]. JOURNAL OF CELL BIOLOGY, 1992, 117 (06) : 1251 - 1261
  • [5] BROOKS SF, 1992, J BIOL CHEM, V267, P14212
  • [6] PURIFICATION AND CHARACTERIZATION OF A MAJOR PHOSPHATIDYLSERINE-BINDING PHOSPHOPROTEIN FROM HUMAN PLATELETS
    BURGENER, R
    WOLF, M
    GANZ, T
    BAGGIOLINI, M
    [J]. BIOCHEMICAL JOURNAL, 1990, 269 (03) : 729 - 734
  • [7] CHAPLINE C, 1993, J BIOL CHEM, V268, P6858
  • [8] THE GROWTH ARREST-SPECIFIC GENE, GAS1, IS INVOLVED IN GROWTH SUPPRESSION
    DELSAL, G
    RUARO, ME
    PHILIPSON, L
    SCHNEIDER, C
    [J]. CELL, 1992, 70 (04) : 595 - 607
  • [9] DELSAL G, 1994, BIOTECHNIQUES, V16, P134
  • [10] A comprehensive genetic map of the human genome based on 5,264 microsatellites
    Dib, C
    Faure, S
    Fizames, C
    Samson, D
    Drouot, N
    Vignal, A
    Millasseau, P
    Marc, S
    Hazan, J
    Seboun, E
    Lathrop, M
    Gyapay, G
    Morissette, J
    Weissenbach, J
    [J]. NATURE, 1996, 380 (6570) : 152 - 154