Developmental expression of stress response genes in Theobroma cacao leaves and their response to Nep1 treatment and a compatible infection by Phytophthora megakarya

被引:44
作者
Bailey, BA
Bae, H
Strem, MD
de Mayolo, GA
Guiltinan, MJ
Verica, JA
Maximova, SN
Bowers, JH
机构
[1] USDA ARS, Sustainable Perennial Crops Lab, Inst Plant Sci, Beltsville Agr Res Ctr W, Beltsville, MD 20705 USA
[2] Penn State Univ, Wartik Lab 306, University Pk, PA 16802 USA
关键词
cacao; developmental expression; Nep1; Phytophthora megakarya; plant defense; susceptibility; Theobroma cacao;
D O I
10.1016/j.plaphy.2005.04.006
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Developmental expression of stress response genes in Theobroma cacao leaves and their response to Nep1 and a compatible infection by Phytophthora megakarya were studied. Ten genes were selected to represent genes involved in defense (TcCaf-1, TcGlu1,3, TcChiB, TcCou-1, and TcPer-1), gene regulation (TcWRKY-1 and TcORFX-1), cell wall development (TcCou-1, TcPer-1, and TcGlu-1), or energy production (TcLhca-1 and TcrbcS). Leaf development was separated into unexpanded (UE), young red (YR), immature green (IG), and mature green (MG). Our data indicates that the constitutive defense mechanisms used by cacao leaves differ between different developmental stages. TcWRKY-1 and TcChiB were highly expressed in MG leaves, and TcPer-1, TcGlu-1, and TcCou-1 were highly expressed in YR leaves. TcGlu1,3 was highly expressed in UE and YR leaves, TcCaf-1 was highly expressed in UE leaves, and TcLhca-1 and TcrbcS were highly expressed in IG and MG leaves. NEP1 encodes the necrosis inducing protein Nep1 produced by Fusarium oxysporum and has orthologs in Phytophthora species. Nep1 caused cellular necrosis on MG leaves and young pods within 24 h of application. Necrosis was observed on YR leaves 10 days after treatment. Expression of TcWRKY-1, TcORFX-1, TcPer-1, and TcGlu-1 was enhanced and TcLhca-1 and TcrbcS were repressed in MG leaves after Nep1 treatment. Expression of TcWRKY-1 and TcORFX-1 was enhanced in YR leaves after Nep1 treatment. Infection of MG leaf disks by P. megakarya zoospores enhanced expression of TcGlu-1, TcWRKY-1, and TcPer-1 and repressed expression of TcChiB, TcLhca-1 and TcrbcS. Five of the six genes that were responsive to Nep1 were responsive to infection by P. megakarya. Susceptibility of T. cacao to P. megakatya includes altered plant gene expression and phytotoxic molecules like Nep1 may contribute to susceptibility. Published by Elsevier SAS.
引用
收藏
页码:611 / 622
页数:12
相关论文
共 56 条
[1]   Engineering hypervirulence in a mycoherbicidal fungus for efficient weed control [J].
Amsellem, Z ;
Cohen, BA ;
Gressel, J .
NATURE BIOTECHNOLOGY, 2002, 20 (10) :1035-1039
[2]   Induction and accumulation of caffeine in young, actively growing leaves of cocoa (Theobroma cacao L.) by wounding or infection with Crinipellis perniciosa [J].
Aneja, M ;
Gianfagna, T .
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 2001, 59 (01) :13-16
[3]   Trends in lignin modification: a comprehensive analysis of the effects of genetic manipulations/mutations on lignification and vascular integrity [J].
Anterola, AM ;
Lewis, NG .
PHYTOCHEMISTRY, 2002, 61 (03) :221-294
[4]   Fungal endophytes limit pathogen damage in a tropical tree [J].
Arnold, AE ;
Mejía, LC ;
Kyllo, D ;
Rojas, EI ;
Maynard, Z ;
Robbins, N ;
Herre, EA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (26) :15649-15654
[5]   Biosynthesis of caffeine in leaves of coffee [J].
Ashihara, H ;
Monteiro, AM ;
Gillies, FM ;
Crozier, A .
PLANT PHYSIOLOGY, 1996, 111 (03) :747-753
[6]   Heterotrimeric and unconventional GTP binding proteins in plant cell signaling [J].
Assmann, SM .
PLANT CELL, 2002, 14 (SUPPL.) :S355-S373
[7]   AIR POLLUTANT STRESS CHANGES THE STEADY-STATE TRANSCRIPT LEVELS OF 3 PHOTOSYNTHESIS GENES [J].
BAHL, A ;
KAHL, G .
ENVIRONMENTAL POLLUTION, 1995, 88 (01) :57-65
[8]   Nep1 protein from Fusarium oxysporum enhances biological control of opium poppy by Pleospora papaveracea [J].
Bailey, BA ;
Apel-Birkhold, PC ;
Akingbe, OO ;
Ryan, JL ;
O'Neill, NR ;
Anderson, JD .
PHYTOPATHOLOGY, 2000, 90 (08) :812-818
[9]  
Bailey BA, 1995, PLANT CELL PHYSIOL, V36, P1669
[10]   Expression of NEP1 by Fusarium oxysporum f. sp erythroxyli after gene replacement and overexpression using polyethylene glycol-mediated transformation [J].
Bailey, BA ;
Apel-Birkhold, PC ;
Luster, DG .
PHYTOPATHOLOGY, 2002, 92 (08) :833-841