Quantitative determination of singlet oxygen generated by excited state aromatic amino acids, proteins, and immunoglobulins

被引：94

作者：

Chin, Khin K. ^{[2
]}

Trevithick-Sutton, Colleen C. ^{[3
]}

McCallum, Jeremy ^{[4
]}

Jockusch, Steffen ^{[1
]}

Turro, Nicholas J. ^{[1
]}

Scaiano, J. C. ^{[3
]}

Foote, Christopher S. ^{[2
]}

Garcia-Garibay, Miguel A. ^{[2
]}

机构：

[1] Columbia Univ, Dept Chem, New York, NY 10027 USA

[2] Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90095 USA

[3] Univ Ottawa, Dept Chem, Ottawa, ON K1N 6N5, Canada

[4] Loyola Marymount Univ, Dept Chem & Biochem, Los Angeles, CA 90045 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2008年 / 130卷 / 22期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1021/ja800926v

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Singlet oxygen quantum yields generated by excited state aromatic amino acids (tryptophan, tyrosine, phenylalanine), N-acetylated amino acids (N-acetyl-tryptophan, N-acetyl-tyrosine, N-acetyl-phenylalanine), and from selected proteins and immunoglobulins have been quantified by time-resolved phosphorescence measurements. A small, but significant, quantum yield found for proteins and immunoglobulins demonstrates that molecular oxygen can diffuse through the polypeptide matrix and can be sensitized by residues buried within the folds of protein structure.

引用

页码：6912 / +

页数：3

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