Characterization of polyclonal antibodies raised against sapovirus genogroup five virus-like particles

被引：13

作者：

Hansman, GS

Natori, K

Ushijima, H

Katayama, K

Takeda, N

机构：

[1] Natl Inst Infect Dis, Dept Virol 2, Tokyo 2080011, Japan

[2] Univ Tokyo, Grad Sch Med, Dept Dev Med Sci, Tokyo, Japan

来源：

ARCHIVES OF VIROLOGY | 2005年 / 150卷 / 07期

关键词：

D O I：

10.1007/s00705-005-0506-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Sapovirus (SaV), a member of the genus Sapovirus in the family Caliciviridae, is an agent of human and porcine gastroenteritis. SaV strains were recently divided into five genogroups (GI to GV). We characterized novel polyclonal antibodies raised against SaV GV virus-like particles (VLPs) by Western blot analysis, and both antibody and antigen enzyme-linked immunosorbent assays (ELISAs). Our results have indicated SaV GI and GV VLPs were antigenically distinct by Western blotting and ELISAs. These reagents may be useful for genogroup specific detection of SaV.

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页码：1433 / 1437

页数：5

相关论文

共 15 条

[1] FECAL SHEDDING OF VIRUS IN RELATION TO THE DAYS OF ILLNESS IN INFANTILE GASTROENTERITIS DUE TO CALICIVIRUS [J].

CHIBA, S ;

SAKUMA, Y ;

KOGASAKA, R ;

AKIHARA, M ;

TERASHIMA, H ;

HORINO, K ;

NAKAO, T .

JOURNAL OF INFECTIOUS DISEASES, 1980, 142 (02) :247-249

[2] Genetic diversity among sapoviruses [J].

Farkas, T ;

Zhong, WM ;

Jing, Y ;

Huang, PW ;

Espinosa, SM ;

Martinez, N ;

Morrow, AL ;

Ruiz-Palacios, GM ;

Pickering, LK ;

Jiang, X .

ARCHIVES OF VIROLOGY, 2004, 149 (07) :1309-1323

[3]

Guntapong R, 2004, JPN J INFECT DIS, V57, P276

[4] Cross-reactivity among sapovirus recombinant capsid proteins [J].

Hansman, GS ;

Natori, K ;

Oka, T ;

Ogawa, S ;

Tanaka, K ;

Nagata, N ;

Ushijima, H ;

Takeda, N ;

Katayama, K .

ARCHIVES OF VIROLOGY, 2005, 150 (01) :21-36

[5] Sapporo-like human caliciviruses are genetically and antigenically diverse [J].

Jiang, X ;

Cubitt, WD ;

Berke, T ;

Zhong, W ;

Dai, X ;

Nakata, S ;

Pickering, LK ;

Matson, DO .

ARCHIVES OF VIROLOGY, 1997, 142 (09) :1813-1827

[6] Diagnosis of human caliciviruses by use of enzyme immunoassays [J].

Jiang, X ;

Wilton, N ;

Zhong, WM ;

Farkas, T ;

Huang, PW ;

Barrett, E ;

Guerrero, M ;

Ruiz-Palacios, G ;

Green, KY ;

Green, J ;

Hale, AD ;

Estes, MK ;

Pickering, LK ;

Matson, DO .

JOURNAL OF INFECTIOUS DISEASES, 2000, 181 :S349-S359

[7] HUMORAL IMMUNITY IN INFANTS WITH GASTROENTERITIS CAUSED BY HUMAN CALICIVIRUS [J].

NAKATA, S ;

CHIBA, S ;

TERASHIMA, H ;

YOKOYAMA, T ;

NAKAO, T .

JOURNAL OF INFECTIOUS DISEASES, 1985, 152 (02) :274-279

[8] DETECTION OF HUMAN CALICIVIRUS ANTIGEN AND ANTIBODY BY ENZYME-LINKED IMMUNOSORBENT ASSAYS [J].

NAKATA, S ;

ESTES, MK ;

CHIBA, S .

JOURNAL OF CLINICAL MICROBIOLOGY, 1988, 26 (10) :2001-2005

[9] MICROTITER SOLID-PHASE RADIOIMMUNOASSAY FOR DETECTION OF HUMAN CALICIVIRUS IN STOOLS [J].

NAKATA, S ;

CHIBA, S ;

TERASHIMA, H ;

SAKUMA, Y ;

KOGASAKA, R ;

NAKAO, T .

JOURNAL OF CLINICAL MICROBIOLOGY, 1983, 17 (02) :198-201

[10]

Noel JS, 1997, J MED VIROL, V52, P173, DOI 10.1002/(SICI)1096-9071(199706)52:2&lt