Plant regeneration from in vitro leaves of mature black cherry (Prunus serotina)

A regeneration system was developed for Prunus serotina from a juvenile (F) and two mature genotypes (#3 and #4). Adventitious shoots regenerated from leaves of in vitro cultures on woody plant medium with thidiazuron (TDZ) and naphthaleneacetic acid (NAA). The best regeneration for genotype F (91.4%) was observed on medium with 9.08 mu M TDZ and 1.07 mu M NAA. The highest mean number of shoots (8.2) was obtained on medium containing 9.08 mu M TDZ and 0.54 mu M NAA. Genotype #3 had the highest regeneration (41.7%) with a mean number of shoots (4.8) on 9.08 mu M TDZ and 1.07 mu M NAA, whereas genotype #4 had a 38.8% regeneration with a mean of 3.3 shoots. Genotype #4 had the highest mean number of shoots (4.8) on 4.54 mu M TDZ and 1.07 mu M NAA. Silver thiosulphate at 60 or 80 mu M increased the percent regeneration of the mature genotypes #3 (75%) and #4 (58%). Adventious shoots were rooted (70-76%) and rooted plantlets survived after acclimatization to the greenhouse. The effect of kanamycin concentration on adventitious shoot regeneration was also evaluated.