The PPR-DYW proteins are required for RNA editing of rps14, cox1 and nad5 transcripts in Physcomitrella patens mitochondria

被引：45

作者：

Uchida, Masato ^{[1
]}

Ohtani, Shotaro ^{[1
]}

Ichinose, Mizuho ^{[1
]}

Sugita, Chieko ^{[1
]}

Sugita, Mamoru ^{[1
]}

机构：

[1] Nagoya Univ, Ctr Gene Res, Chikusa Ku, Nagoya, Aichi 4648602, Japan

来源：

FEBS LETTERS | 2011年 / 585卷 / 14期

基金：

日本学术振兴会;

关键词：

DYW domain; Mitochondrion; Physcomitrella patens; PPR protein; RNA editing; PENTATRICOPEPTIDE REPEAT PROTEIN; ARABIDOPSIS-THALIANA; CHLOROPLASTS; SITES; REVEALS; DOMAIN; PLANTS;

D O I：

10.1016/j.febslet.2011.06.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We identified two DYW subclass pentatricopeptide repeat (PPR) proteins, PpPPR_78 and PpPPR_79, as RNA editing factors in the moss Physcomitrella patens. Disruption of each gene by homologous recombination revealed that PpPPR_78 was involved in RNA editing at the rps14 (rps14-C137) and cox1 (cox1-C755) sites and PpPPR_79 at the nad5-1 (nad5-C598) site in the mitochondrial transcripts. RNA editing defects did not affect transcript patterns of the target genes. Thus, DYW subclass PPR proteins seem to be site-specific trans-acting factors for RNA editing. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：2367 / 2371

页数：5

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