Characteristic genotypes discriminate between Babesia canis isolates of differing vector specificity and pathogenicity to dogs

被引：169

作者：

Zahler, M

Schein, E

Rinder, H

Gothe, R

机构：

[1] Inst Vergleichende Tropenmed & Parasitol, D-80802 Munich, Germany

[2] Free Univ Berlin, Inst Parasitol & Tropenveterinarmed, D-14163 Berlin, Germany

[3] LMU, Klinikum Innenstadt, Abt Infekt & Tropenmed, D-80802 Munich, Germany

来源：

PARASITOLOGY RESEARCH | 1998年 / 84卷 / 07期

关键词：

Polymerase Chain Reaction; Code Region; Species Level; Canis; Geographic Origin;

D O I：

10.1007/s004360050445

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The first and second internal transcribed spacers (ITS1, ITS2) as well as the intervening 5.8S coding region of the rRNA gene were characterized in eight Babesia canis isolates of differing geographic origin, vector specificity; and pathogenicity to dogs. The genotypes determined by sequencing segregated into three clearly separated groups close to or near the species level and correspond to the previously proposed subspecies B. canis canis, B. canis vogeli, and B. canis rossi. The three genotypes can be distinguished by Sau96I digestion of the polymerase chain reaction (PCR)-amplified rDNA target.

引用

页码：544 / 548

页数：5

共 15 条

[1]

BRUMPT E., 1938, ANN PARASITOL HUMAINE ET COMP, V16, P97

[2]

CITARD T, 1995, TROP MED PARASITOL, V46, P172

[3]

DEWAAL DT, 1990, ONDERSTEPOORT J VET, V57, P99

[4]

GLASER B, 1998, HUNDETOURISMUS IMPRO