Mechanical regulation of cell function with geometrically modulated elastomeric substrates

被引：848

作者：

Fu, Jianping ^{[1
]}

Wang, Yang-Kao ^{[1
]}

Yang, Michael T. ^{[1
]}

Desai, Ravi A. ^{[1
]}

Yu, Xiang ^{[1
]}

Liu, Zhijun ^{[1
]}

Chen, Christopher S. ^{[1
]}

机构：

[1] Univ Penn, Dept Bioengn, Philadelphia, PA 19104 USA

来源：

NATURE METHODS | 2010年 / 7卷 / 09期

关键词：

FOCAL ADHESIONS; LINEAGE; FORCES; MATRIX;

D O I：

10.1038/nmeth.1487

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the establishment of a library of micromolded elastomeric micropost arrays to modulate substrate rigidity independently of effects on adhesive and other material surface properties. We demonstrated that micropost rigidity impacts cell morphology, focal adhesions, cytoskeletal contractility and stem cell differentiation. Furthermore, early changes in cytoskeletal contractility predicted later stem cell fate decisions in single cells.

引用

收藏

页码：733 / U95

页数：7

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