Respiratory syncytial virus influences NF-κB-dependent gene expression through a novel pathway involving MAP3K14/NIK expression and nuclear complex formation with NF-κB2

A member of the Paramyxoviridae family of RNA viruses, respiratory syncytial virus (RSV), is a leading cause of epidemic respiratory tract infection in children. In children, RSV primarily replicates in the airway mucosa, a process that alters epithelial cell chemokine expression, thereby inducing airway inflammation. We investigated the role of the mitogen-activated protein kinase kinase kinase 14/NF-kappa B-inducing kinase (NIK) in the activation of NF-kappa B-dependent genes in alveolus-like A549 cells. RSV infection induces a time dependent increase of NIK mRNA and protein expression that peaks 12 to 24 h after viral exposure. Immunoprecipitation kinase assays indicate that NIK kinase activity is activated even more rapidly (within 6 h of RSV adsorption) associated with an endogenous similar to 50-kDa NF-kappa B2 substrate. Because NIK associates with IKK alpha to mediate processing of the 100-kDa NF-kappa B2 precursor into its 52-kDa DNA binding isoform ("p52"), the effects of RSV on NIK complex formation with IKKa and NF-kappa B2 were determined by coimmunoprecipitation assay. We find that NIK, IKKa, and both 100 kDa- and 52-kDa NF-kappa B2 isoforms strongly complex 15 h after exposure to RSV at times subsequent to NIK kinase activation. Western immunoblot and microaffinity DNA pull-down assays showed a parallel increase in nuclear translocation and DNA binding of the NF-kappa B2-Re1 B complex. Interestingly, we make the novel observations that NIK also transiently translocates into the nucleus complexed with 52-kDa NF-kappa B2. Small interfering RNA-mediated NIK "knock-down" blocked RSV-inducible 52-kDa NF-kappa B2 processing and interfered with the early activation of a subset of NF-kappa B-dependent genes, indicating the importance of this activation pathway in the genomic NF-kappa B response to RSV. Together, these data indicate that RSV infection rapidly activates the noncanonical NF-kappa B activation pathway prior to the more potent canonical pathway activation. This appears to be through a novel mechanism involving induction of NIK kinase activity, expression, and nuclear translocation of a ternary complex with IKK alpha and processed NF-kappa B2.