Isolation and cytotoxic properties of a novel glycoconjugate from corms of saffron plant (Crocus sativus L.)

被引：34

作者：

Escribano, J ^{[1
]}

Ríos, I ^{[1
]}

Fernández, JA ^{[1
]}

机构：

[1] Univ Castilla La Mancha, Secc Biotecnol, Inst Desarrollo Reg, E-02071 Albacete, Spain

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 1999年 / 1426卷 / 01期

关键词：

cytotoxicity; glycoconjugate; proteoglycan; saffron plant; (Crocus sativus);

D O I：

10.1016/S0304-4165(98)00149-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A novel glycoconjugate isolated from saffron corm extracts shows remarkable cytotoxic activity on cultured human cancer cells (HeLa). Its polysaccharide part represents 94.5% of the molecule, and is dominated by a 36.4% of rhamnose. The protein backbone is composed mainly of aspartic acid/asparagine, alanine, glutamic acid/glutamine, glycine and serine. HeLa cells exposed to this glycoconjugate showed swelling and local plasma membrane evaginations, suggesting that cytotoxicity is mediated by extracellular fluid uptake. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：217 / 222

页数：6

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