At line monitoring of bioreactor protein production by surface plamon resonance

被引：16

作者：

Jacquemart, R. ^{[1
]}

Chavane, N. ^{[1
]}

Durochcr, Y. ^{[2
]}

Hoemann, C. ^{[1
]}

De Crescenzo, C. ^{[1
]}

Jolicoeur, M. ^{[1
]}

机构：

[1] Ecole Polytech, Dept Chem Engn, Montreal, PQ H3C 3A7, Canada

[2] Biotechnol Res Inst NRC, Montreal, PQ, Canada

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 2008年 / 100卷 / 01期

关键词：

at-line protein monitoring; bioreactor protein production; HEK; 293; cells; surface plasmon resonance; transient transfection;

D O I：

10.1002/bit.21725

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An innovative and automated method for the at-line monitoring of secreted protein was developed by harnessing a Surface Plasmon Resonance-based biosensor to a bioreactor. The proof of concept was performed by following at-line the relative concentration of a secreted protein produced by transient transfection of mammalian cells in a bioreactor. Our results suggest that our approach can be readily applied to the at-line determination of both protein concentration and bioactivity. Our experimental setup and strategy can thus satisfy the needs related to the development of novel bioprocess control protocols in the context of the new process analytical technology that arises in the biopharmaceutical industry.

引用

页码：184 / 188

页数：5

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