Assessment of cytotoxicity of quantum dots and gold nanoparticles using cell-based. impedance spectroscopy

被引：136

作者：

Male, Keith B. ^{[1
]}

Lachance, Bernard ^{[1
]}

Hrapovic, Sabahudin ^{[1
]}

Sunahara, Geoff ^{[1
]}

Luong, John H. T. ^{[1
,2
]}

机构：

[1] Natl Res Council Canada, Biotechnol Res Inst, Montreal, PQ H4P 2R2, Canada

[2] Natl Univ Ireland Univ Coll Cork, Dept Chem, Cork, Ireland

来源：

ANALYTICAL CHEMISTRY | 2008年 / 80卷 / 14期

关键词：

D O I：

10.1021/ac8004555

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A continuous online technique based on electric cell-substrate impedance sensing (ECIS) was demonstrated for measuring the concentration and time response function of fibroblastic V79 cells exposed to nanomaterials such as quantum dots (QDs) and fluorescent gold nanoparticles. The half-inhibition concentration, (ECIS50), the required concentration to attain 50% inhibition of the cytotoxic response, was estimated from the response function to ascertain cytotoxicity during the course of measurement. The ECIS50 values agreed well with the results obtained using the standard neutral red assay. Cadmium selenide quantum dots showed direct cytoxicity with the ECIS assay. For the cadmium telluride quantum dots, significant toxicity. could be assigned to free cadmium, although additional toxicity could be attributed to the QDs per se. The QDs synthesized with indium gallium phosphide and the fluorescent gold nanoparticles were not cytotoxic.

引用

页码：5487 / 5493

页数：7

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