Virulence of pigeon-origin Newcastle disease virus isolates for domestic chickens

被引:108
作者
Kommers, GD
King, DJ
Seal, BS
Brown, CC
机构
[1] USDA ARS, SE Poultry Res Lab, Athens, GA 30605 USA
[2] Univ Georgia, Coll Vet Med, Dept Pathol, Athens, GA 30602 USA
关键词
chickens; in situ hybridization; immunohistochemistry; sequence analysis; pigeon paramyxovirus-1; avian paramyxovirus-1; Newcastle disease; virulence;
D O I
10.2307/1592870
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
The virulence of six pigeon-origin isolates of Newcastle disease virus (NDV) was evaluated before and after passage in white leghorn chickens. Four isolates were defined as pigeon paramyxovirus-1 (PPMV-1) and two isolates were classified as avian paramyxovirus-1 (APMV-1) with NDV monoclonal antibodies. The four PPMV-1 isolates were passaged four times in chickens, and the APMV-1 isolates were passaged only once. Infected birds were monitored clinically and euthanatized. Tissues were collected fur histopathology, in situ hybridization with a NDV matrix gene digoxigenin-labeled riboprobe. and immunohistochemistry with an anti-peptide antibody to the nucleoprotein, Mean death time, intracerebral pathogenicity index, and intravenous pathogenicity index tests performed before a-rid after passage in chickens demonstrated increased virulence Of the passaged PPMV-1 isolates and high virulence of the original isolates of AFMV-1. Sequence analysis of the fusion protein cleavage site of all six isolates demonstrated a sequence typical of the virulent pathotype, Although the pathotyping results indicated a virulence increase of dl passaged PPMV-1 isolates, clinical disease was limited to depression and some nervous signs in only some of the 4-wk-old specific-pathogen-free white leghorns inoculated intraconjunctivally. However, an increased frequency of clinical signs and some mortality occurred in 2 wk Olds inoculated intraconjunctivally with passaged virus. Histologically, prominent lesions in heart and brain were observed in birds among all four groups inoculated with the PPMV-1 isolates. The behavior of the two pigeon-origin APMV-1 isolates when inoculated into chickens was characteristic of velogenic viscerotropic NDVs and included necro-hemorrhagic lesions in the gastrointestinal tract.
引用
收藏
页码:906 / 921
页数:16
相关论文
共 48 条
[1]  
Alexander D., 1985, P 34 ANN W POULTR C, P131
[2]  
Alexander D.J., 1997, Diseases of poultry, V10th, P541
[3]  
Alexander D.J., 1998, A laboratory manual for the isolation and identification of avian pathogens, V4th, P156
[4]   INFECTION OF FOWLS WITH NEWCASTLE-DISEASE VIRUS BY FOOD CONTAMINATED WITH PIGEON FECES [J].
ALEXANDER, DJ ;
PARSONS, G ;
MARSHALL, R .
VETERINARY RECORD, 1984, 115 (23) :601-602
[5]   AVIAN PARAMYXOVIRUS TYPE-1 INFECTIONS OF RACING PIGEONS - 2 PATHOGENICITY EXPERIMENTS IN PIGEONS AND CHICKENS [J].
ALEXANDER, DJ ;
PARSONS, G .
VETERINARY RECORD, 1984, 114 (19) :466-469
[6]   PARAMYXOVIRUS TYPE-1 INFECTIONS OF RACING PIGEONS .1. CHARACTERIZATION OF ISOLATED VIRUSES [J].
ALEXANDER, DJ ;
RUSSELL, PH ;
COLLINS, MS .
VETERINARY RECORD, 1984, 114 (18) :444-446
[7]   NEWCASTLE-DISEASE OUTBREAKS IN FOWL IN GREAT-BRITAIN DURING 1984 [J].
ALEXANDER, DJ ;
WILSON, GWC ;
RUSSELL, PH ;
LISTER, SA ;
PARSONS, G .
VETERINARY RECORD, 1985, 117 (17) :429-434
[8]   PATHOGENICITY FOR CHICKENS OF AVIAN PARAMYXOVIRUS TYPE-1 ISOLATES OBTAINED FROM PIGEONS IN GREAT-BRITAIN DURING 1983-85 [J].
ALEXANDER, DJ ;
PARSONS, G .
AVIAN PATHOLOGY, 1986, 15 (03) :487-493
[9]   AVIAN PARAMYXOVIRUS TYPE-1 INFECTION OF RACING PIGEONS .3. EPIZOOTIOLOGICAL CONSIDERATIONS [J].
ALEXANDER, DJ ;
WILSON, GWC ;
THAIN, JA ;
LISTER, SA .
VETERINARY RECORD, 1984, 115 (09) :213-216
[10]   CHEMICAL COUPLING OF PEPTIDES AND PROTEINS TO POLYSACCHARIDES BY MEANS OF CYANOGEN HALIDES [J].
AXEN, R ;
PORATH, J ;
ERNBACK, S .
NATURE, 1967, 214 (5095) :1302-&