Evaluation of two media for the membrane filtration enumeration of Clostridium perfringens from water

被引：21

作者：

Sartory, DP ^{[1
]}

Field, M ^{[1
]}

Curbishley, SM ^{[1
]}

Pritchard, AM ^{[1
]}

机构：

[1] Severn Trent Water Qual & Environm Serv, Shrewsbury SY3 8BJ, Salop, England

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1998年 / 27卷 / 06期

关键词：

D O I：

10.1046/j.1472-765X.1998.00454.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

TWO media (mCP medium and Tryptose Sulphite Cycloserine (TSC) agar) were evaluated for recovery of Clostridium perfringens in environmental and part-treated drinking water. For laboratory strains of Clostridium, mCP was more selective and specific for CI. perfringens than TSC, but was markedly less efficient for the enumeration of both vegetative cells and spores. For samples of river water and part-treated drinking water, TSC recovered significantly greater numbers of Cl. perfringens than mCP. In contrast to previous reports, there was a significant number of false presumptive positive and negative isolates on mCP. TSC is a more suitable medium for the routine monitoring of water supplies for the presence of CI. perfringens.

引用

页码：323 / 327

页数：5

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