Nanoengineering the Heart: Conductive Scaffolds Enhance Connexin 43 Expression

被引：252

作者：

You, Jin-Oh ^{[1
]}

Rafat, Marjan ^{[1
]}

Ye, George J. C. ^{[1
]}

Auguste, Debra T. ^{[1
]}

机构：

[1] Harvard Univ, Sch Engn & Appl Sci, Cambridge, MA 02138 USA

来源：

NANO LETTERS | 2011年 / 11卷 / 09期

基金：

美国国家科学基金会;

关键词：

Scaffold; cardiomyocyte; conductive; Au nanoparticles; HEMA; connexin; 43; ELECTRICAL-STIMULATION; GOLD NANOPARTICLES; CARDIAC MYOCYTES; IN-VITRO; MECHANICAL-PROPERTIES; CHITOSAN SCAFFOLDS; SURFACE-DENSITY; TISSUE; POLYPYRROLE; STIFFNESS;

D O I：

10.1021/nl201514a

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

Scaffolds that couple electrical and elastic properties may be valuable for cardiac cell function. However, existing conductive materials do not mimic physiological properties. We prepared and characterized a tunable, hybrid hydrogel scaffold based on Au nanoparticles homogeneously synthesized throughout a polymer templated gel. Conductive gels had Young's moduli more similar to myocardium relative to polyaniline and polypyrrole, by 1-4 orders of magnitude. Neonatal rat cardiomyocytes exhibited increased expression of connexin 43 on hybrid scaffolds relative to HEMA with or without electrical stimulation.

引用

页码：3643 / 3648

页数：6

共 67 条

[1]

Efferent vagal nerve stimulation protects heart against ischemia-induced arrhythmias by preserving connexin43 protein [J].

Ando, M ;

Katare, RG ;

Kakinuma, Y ;

Zhang, DM ;

Yamasaki, F ;

Muramoto, K ;

Sato, T .

CIRCULATION, 2005, 112 (02) :164-170

[2]

Influence of Substrate Stiffness on the Phenotype of Heart Cells [J].

Bhana, Bashir ;

Iyer, Rohin K. ;

Chen, Wen Li Kelly ;

Zhao, Ruogang ;

Sider, Krista L. ;

Likhitpanichkul, Morakot ;

Simmons, Craig A. ;

Radisic, Milica .

BIOTECHNOLOGY AND BIOENGINEERING, 2010, 105 (06) :1148-1160

[3]

Black LD, 2009, TISSUE ENG PT A, V15, P3099, DOI [10.1089/ten.tea.2008.0502, 10.1089/ten.TEA.2008.0502]

[4]

Mechanical properties and remodeling of hybrid cardiac constructs made from heart cells, fibrin, and biodegradable, elastomeric knitted fabric [J].

Boublik, J ;

Park, H ;

Radisic, M ;

Tognana, E ;

Chen, F ;

Pei, M ;

Vunjak-Novakovic, G ;

Freed, LE .

TISSUE ENGINEERING, 2005, 11 (7-8) :1122-1132

[5]

Temporal changes in expression of connexin 43 after load-induced hypertrophy in vitro [J].

Bupha-Intr, Tepmanas ;

Haizlip, Kaylan M. ;

Janssen, Paul M. L. .

AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2009, 296 (03) :H806-H814

[6]

CANNIZZARO C, 2008, PRINCIPLES TISSUE EN

[7]

Cell spreading and focal adhesion dynamics are regulated by spacing of integrin ligands [J].

Cavalcanti-Adam, Elisabetta Ada ;

Volberg, Tova ;

Micoulet, Alexandre ;

Kessler, Horst ;

Geiger, Benjamin ;

Spatz, Joachim Pius .

BIOPHYSICAL JOURNAL, 2007, 92 (08) :2964-2974

[8]

Current-Controlled Electrical Point-Source Stimulation of Embryonic Stem Cells [J].

Chen, Michael Q. ;

Xie, Xiaoyan ;

Wilson, Kitchener D. ;

Sun, Ning ;

Wu, Joseph C. ;

Giovangrandi, Laurent ;

Kovacs, Gregory T. A. .

CELLULAR AND MOLECULAR BIOENGINEERING, 2009, 2 (04) :625-635

[9]

Characterisation of a soft elastomer poly(glycerol sebacate) designed to match the mechanical properties of myocardial tissue [J].

Chen, Qi-Zhi ;

Bismarck, Alexander ;

Hansen, Ulrich ;

Junaid, Sarah ;

Tran, Michael Q. ;

Harding, Sian E. ;

Ali, Nadire N. ;

Boccaccini, Aldo R. .

BIOMATERIALS, 2008, 29 (01) :47-57

[10]

Elucidating the mechanism of cellular uptake and removal of protein-coated gold nanoparticles of different sizes and shapes [J].

Chithrani, B. Devika ;

Chan, Warren C. W. .

NANO LETTERS, 2007, 7 (06) :1542-1550

← 1 2 3 4 5 6 7 →