Hybrid hydrogels self-assembled from graft copolymers containing complementary β-sheets as hydroxyapatite nucleation scaffolds

被引：54

作者：

Wu, Larisa C.

Yang, Jiyuan ^{[2
]}

Kopecek, Jindrich ^{[1
,2
]}

机构：

[1] Univ Utah, Ctr Controlled Drug Delivery, Dept Bioengn, Salt Lake City, UT 84112 USA

[2] Univ Utah, Dept Pharmaceut & Pharmaceut Chem, Salt Lake City, UT 84112 USA

来源：

BIOMATERIALS | 2011年 / 32卷 / 23期

关键词：

Graft copolymers; HPMA; beta-Sheet peptides; Self-assembly; Scaffold; Biomineralization; BONE; PEPTIDE; MINERALIZATION; BIOMINERALIZATION; POLYMERS; FIBRILS; SURFACE; DESIGN;

D O I：

10.1016/j.biomaterials.2011.04.014

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

A biomimetic material that can assist bone tissue regeneration was proposed. A bone scaffold based on a hybrid hydrogel self-assembled from N-(2-hydroxypropyl)methacrylamide (HPMA) copolymers grafted with complementary beta-sheet peptides was designed. Investigation of self-assembly by circular dichroism spectroscopy suggested that hydrogel formation was triggered through association of the complementary beta-sheet motifs. Congo Red and thioflavin T binding, as well as transmission electron microscopy confirmed the formation of a fibril network. Besides mimicking the natural bone extracellular matrix and maintaining preosteoblast cells viability, this hydrogel, as shown by scanning electron microscopy and Fourier transform infrared spectroscopy, provided surfaces characterized by epitaxy that favored hydroxyapatite-like crystal nucleation and growth potentially beneficial for biointegration. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：5341 / 5353

页数：13

共 54 条

[1] pH as a trigger of peptide β-sheet self-assembly and reversible switching between nematic and isotropic phases [J].