Requirement for p53 and p21 to sustain G2 arrest after DNA damage

被引：2597

作者：

Bunz, F

Dutriaux, A

Lengauer, C

Waldman, T

Zhou, S

Brown, JP

Sedivy, JM

Kinzler, KW

Vogelstein, B

机构：

[1] Johns Hopkins Oncol Ctr, Howard Hughes Med Inst, Baltimore, MD 21231 USA

[2] Brown Univ, Dept Biochem Mol Biol & Cell Biol, Div Biol & Med, Providence, RI 02912 USA

[3] Johns Hopkins Univ, Sch Med, Program Human Genet, Baltimore, MD 21205 USA

[4] Johns Hopkins Univ, Sch Med, Johns Hopkins Oncol Ctr, Baltimore, MD 21205 USA

来源：

SCIENCE | 1998年 / 282卷 / 5393期

关键词：

D O I：

10.1126/science.282.5393.1497

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

After DNA damage, many cells appear to enter a sustained arrest in the G(2) phase of the cell cycle. It is shown here that this arrest could be sustained only when p53 was present in the cell and capable of transcriptionally activating the cyclin-dependent kinase inhibitor p21. After disruption of either the p53 or the p21 gene, gamma radiated cells progressed into mitosis and exhibited a G(2) DNA content only because of a failure of cytokinesis. Thus, p53 and p21 appear to be essential for maintaining the G(2) checkpoint in human cells.

引用

页码：1497 / 1501

页数：5

共 48 条

[1] P53 CONTROLS BOTH THE G(2)/M AND THE G(1) CELL-CYCLE CHECKPOINTS AND MEDIATES REVERSIBLE GROWTH ARREST IN HUMAN FIBROBLASTS [J].