Preparation of oligonucleotides containing 5-bromouracil and 5-methylcytidine.

被引：16

作者：

Ferrer, E

Fabrega, C

Garcia, RG

Azorin, F

Eritja, R

机构：

[1] CSIC,CTR INVEST & DESARROLLO,E-08034 BARCELONA,SPAIN

[2] EUROPEAN MOLEC BIOL LAB,D-69117 HEIDELBERG,GERMANY

来源：

NUCLEOSIDES & NUCLEOTIDES | 1996年 / 15卷 / 04期

关键词：

D O I：

10.1080/07328319608002137

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A previously described side reaction on 5-bromouracil during standard oligonucleotide deprotection conditions has been studied in detail. The side product, 5-amino-2'-deoxyuridine, is isolated and characterized. The use of several 5-methylcytidine protected derivatives for the preparation of oligonucleotides containing 5-bromouracil and 5-methylcytidine free of 5-amino-2'-deoxyuridine is discussed.

引用

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页码：907 / 921

页数：15

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