Stable in vivo imaging of densely populated glia, axons and blood vessels in the mouse spinal cord using two-photon microscopy

被引：89

作者：

Davalos, Dimitrios ^{[1
]}

Lee, Jae K. ^{[2
]}

Smith, W. Bryan ^{[3
]}

Brinkman, Brendan ^{[2
]}

Ellisman, Mark H. ^{[2
,3
]}

Zheng, Binhai ^{[2
]}

Akassoglou, Katerina ^{[1
]}

机构：

[1] Univ Calif San Diego, Dept Pharmacol, La Jolla, CA 92093 USA

[2] Univ Calif San Diego, Dept Neurosci, La Jolla, CA 92093 USA

[3] Univ Calif San Diego, Natl Ctr Microscop & Imaging Res, La Jolla, CA 92093 USA

来源：

JOURNAL OF NEUROSCIENCE METHODS | 2008年 / 169卷 / 01期

关键词：

spinal cord; microglia; axons; in vivo imaging; two-photon microscopy;

D O I：

10.1016/j.jneumeth.2007.11.011

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In vivo imaging has revolutionized our understanding of biological processes in brain physiology and pathology. However, breathing-induced movement artifacts have impeded the application of this powerful too] in studies of the living spinal cord. Here we describe in detail a method to image stably and repetitively, using two-photon microscopy, the living spinal tissue in mice with dense fluorescent cells or axons, without the need for animal intubation or image post-processing. This simplified technique can greatly expand the application of in vivo imaging to study spinal cord injury, regeneration, physiology and disease. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：1 / 7

页数：7

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