Brown adipose tissue mitochondrial subpopulations show different morphological and thermogenic characteristics

被引：34

作者：

Justo, R ^{[1
]}

Oliver, J ^{[1
]}

Gianotti, M ^{[1
]}

机构：

[1] Univ Illes Balears, IUNICS, Dept Biol Fonamental & Ciencies Salut, Grp Metab Energet & Nutr, E-07122 Palma de Mallorca, Spain

来源：

MITOCHONDRION | 2005年 / 5卷 / 01期

关键词：

mitochondrial morphology; mitochondrial DNA; mitochondrial integrity; UCP1; content; antioxidant enzymes; subcellular contamination;

D O I：

10.1016/j.mito.2004.09.003

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Rat brown adipose tissue mitochondrial subpopulations-isolated by differential centrifugation at 1000, 3000 and 8000g, giving the heavy, medium and light mitochondria-were characterized. Thus, contamination by non-mitochondrial subcellular components, morphological features, respiratory chain and antioxidant enzyme activities, both uncoupling protein I and mitochondrial protein content, mitochondrial DNA levels and mitochondrial integrity were measured. Results indicate that mitochondrial fractions showed important differences in the morphological, thermogenic and antioxidant properties. All the parameters studied were always higher in heavy mitochondria, which is indicative of a greater mitochondrial differentiation state. (C) 2004 Elsevier B.V. and Mitochondria Research Society. All rights reserved.

引用

页码：45 / 53

页数：9

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