Antibody W7C5 defines a CD109 epitope expressed on CD34+ and CD34- hematopoietic and mesenchymal stem cell subsets

被引：26

作者：

Giesert, C

Marxer, A

Sutherland, DR

Schuh, AC

Kanz, L

Bühring, HJ

机构：

[1] Univ Tubingen, Med Klin 2, Dept Internal Med 2, Div Hematol Immunol & Oncol, D-72076 Tubingen, Germany

[2] Univ Toronto, Princess Margaret Hosp, Toronto, ON, Canada

来源：

HEMATOPOIETIC STEM CELLS 2002: GENETICS AND FUNCTION | 2003年 / 996卷

关键词：

antibody; CD109; stem cells; CD34(+) stem cells; CD34(-) stem cells; PI-PLC; GPI-linked molecule;

D O I：

10.1111/j.1749-6632.2003.tb03250.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The cell surface antigen recognized by monoclonal antibody W7C5 is expressed at low levels on human CD34(+) and CD34(-) bone marrow stem cell populations but at high levels on fetal liver CD34(+) cells. To identify the recognized antigen, we performed partial amino acid sequence and MALDI analysis of purified W7C5 antigens. The analyses revealed that MoAb W7C5 detects CD109, a recently cloned glycophosphatidylinositol-linked glycoprotein. The specificity of MoAb W7C5 was further confirmed by the selective recognition of CHO cells transfected with human CD109 cDNA.

引用

页码：227 / 230

页数：4

共 7 条

[1]

Giesert C, 2001, ANN NY ACAD SCI, V938, P175

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