Electron density modeling and reconstruction of infinite periodic minimal surfaces (IPMS) based phases in lipid-water systems. II. Reconstruction of D surface based phases

被引:19
作者
Harper, PE
Gruner, SM
Lewis, RNAH
McElhaney, RN
机构
[1] Princeton Univ, Joseph Henry Labs, Dept Phys, Princeton, NJ 08544 USA
[2] Univ Alberta, Dept Biochem, Edmonton, AB T6G 2H7, Canada
关键词
D O I
10.1007/PL00013664
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
This is the second of two papers dealing with the structure of lipid-water phases based on Infinite Periodic Minimal Surfaces (IPMS). The first paper describes mathematical modeling of such phases. In this paper, a new reconstruction method, called the methyl trough search, is described and used to solve the structures based on powder pattern X-ray diffraction data. Structures are derived for both a single chain lipid-water system (mono-olein) and a diacyl phospholipid-water system (2-2 methyl butyl 16:0 phosphatidylcholine). The methyl trough search uses the low electron density of the lipid methyl tails to determine the correct phasing for the electron density reconstruction. The data are consistent with a structure based on the IPMS D surface. The results are compared to other methods used to solve the mono-olein structure; the structure of the diacyl lipid has never before been solved. We discuss the subtleties involved in reconstruction of D surface based phases and the substantial artifacts that arise in low-resolution reconstructions of hydrocarbon lipids lacking heavy-atom sites.
引用
收藏
页码:229 / 245
页数:17
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