Up-regulation of endothelial nitric oxide synthase through β2-adrenergic receptor -: The role of a β-blocker with NO-releasing action

被引：22

作者：

Jayachandran, M

Hayashi, T

Sumi, D

Thakur, NK

Kano, H

Ignarro, LJ

Iguchi, A

机构：

[1] Nagoya Univ, Grad Sch Med, Dept Geriatr, Showa Ku, Nagoya, Aichi 4668550, Japan

[2] Univ Calif Los Angeles, Sch Med, Dept Mol & Med Pharmacol, Los Angeles, CA 90095 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2001年 / 280卷 / 03期

关键词：

endothelial nitric oxide synthase; caveolin-1; beta(2)-adrenergic receptor antagonist; nitric oxide;

D O I：

10.1006/bbrc.2000.4177

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We determined the existence and role of beta (2)-adrenergic receptor in cultured BAECs through the effect of a beta -blocker having NO releasing action; 3,4-dihydro-8 (2-hydroxy-3-isopropylamino)-propoxy-3-nitroxy-2H-1-benzopyran; nipradilol on eNOS and eNOS regulatory protein caveolin-1. beta (2) receptor exists in BAECs. eNOS mRNA and protein were up-regulated by its treatment whereas those of caveolin were not altered considerably. This eNOS up-regulatory action was abolished by beta (2) receptor antagonist, ICI-118551. Increase of NO metabolites, protein and mRNA of eNOS was also partially inhibited by co-treatment of NOS inhibitor, L-NA with nipradilol. This is the first investigation of the action of non-selective beta blocker on eNOS through beta (2) receptor. The drug increases NO on incubation with BAECs about 50% as a NO donor and about 50% as results of eNOS up-regulation. (C) 2001 Academic Press.

引用

页码：589 / 594

页数：6

共 22 条

[1]

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[2] Endothelial nitric oxide synthase targeting to caveolae - Specific interactions with caveolin isoforms in cardiac myocytes and endothelial cells [J].