The diagnosis of azoospermia depends on the force of centrifugation

被引:27
作者
Corea, M
Campagnone, J
Sigman, M
机构
[1] Brown Univ, Sch Med, Dept Surg, Div Urol, Providence, RI USA
[2] Rhode Isl Hosp, Dept Urol, Providence, RI USA
关键词
infertility; azoospermia; centrifugation;
D O I
10.1016/j.fertnstert.2004.09.028
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Objective: To determine the centrifugal force required to pellet sperm. Design: Prospective, in vitro study. Setting: Tertiary referral center. Patient(s): Men undergoing semen processing or postvasectomy semen analyses. Intervention(s): In Phase I, postvasectomy semen samples were centrifuged at 600 X g, and the pellets were examined. Supernatants from samples with no visible sperm underwent repeat centrifugation at 1,000 X g; the supernatants were removed and centrifuged at 3,000 X g. Pellets from both centrifugations were examined. Phase II examined nonazoospermic semen that was divided into 3 aliquots and centrifuged at 500, 1,000, and 3,000 X g. The supernatants were examined for the presence of sperm. Main Outcome Measure(s): Sperm presence in centrifuged pellets or seminal supernatant. Result(s): Phase I: After centrifugation at 1,000 X g and 3,000 X g, sperm were noted in 12% and 0% of samples, respectively. Phase II: Sperm were noted in the supernatant in 100% of samples subjected to 500 X g and 1,000 X g, and in 92% of samples subjected to 3,000 X g centrifugation. Conclusion(s): If sperm are in the seminal plasma, they will also be present in the pellet following centrifugation at a force of 1,000 X g or greater. Semen samples that appear azoospermic upon initial wet mount microscopy should be centrifuged at a minimum of 1,000 X g for 15 minutes.
引用
收藏
页码:920 / 922
页数:3
相关论文
共 6 条
  • [1] *AM UR ASS AM SOC, 2001, REP EV AZ MAL
  • [2] FREUND MJ, 1989, J ANDROL, V10, P120
  • [3] Sperm pellet analysis: A technique to detect the presence of sperm in men considered to have azoospermia by routine semen analysis
    Jaffe, TM
    Kim, ED
    Hoekstra, TH
    Lipshultz, LI
    [J]. JOURNAL OF UROLOGY, 1998, 159 (05) : 1548 - 1550
  • [4] KEEL BA, 1990, CRC HDB LAB DIAGNOSI, P49
  • [5] Meacham RB, 2003, J ANDROL, V24, P494
  • [6] SHEKARRIZ M, 1995, EUR UROL, V28, P31