Hyperspectral Raman imaging of bone growth and regrowth chemistry

被引：12

作者：

Pezzuti, JA ^{[1
]}

Morris, MD ^{[1
]}

Bonadio, JF ^{[1
]}

Goldstein, SK ^{[1
]}

机构：

[1] Univ Michigan, Dept Chem, Ann Arbor, MI 48109 USA

来源：

THREE-DIMENSIONAL AND MULTIDIMENSIONAL MICROSCOPY: IMAGE ACQUISITION AND PROCESSING V, PROCEEDINGS OF | 1998年 / 3261卷

关键词：

Raman imaging; hydroxyapatite; factor analysis; bone growth;

D O I：

10.1117/12.310566

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Hyperspectral Raman microscopic imaging of carbonated hydroxyapatite (HAP) is used to follow the chemistry of bone growth and regrowth. Deep red (785 nn) excitation is employed to minimize protein fluorescence interference. A passive line generator based on Powell lens optics and a motorized translation stage provide the imaging capabilities. Raman image contrast is generated from several lines of the HAP Raman spectrum, primarily the PO4-3 stretch at ca. 960 cm(-1). Factor analysis is used to minimize the integration time needed for acceptable contrast and to explore the chemical species within the bone. Bone age is visualized as variations in image intensity. High definition, high resolution images of newly formed bone and mature bone are compared qualitatively. The technique is currently under evaluation for study of experimental therapies for fracture repair.

引用

页码：270 / 276

页数：7