Induced pluripotency: history, mechanisms, and applications

被引：568

作者：

Stadtfeld, Matthias ^{[1
,2
,3
,4
,5
,6
]}

Hochedlinger, Konrad ^{[1
,2
,3
,4
,5
,6
]}

机构：

[1] Harvard Univ, Howard Hughes Med Inst, Cambridge, MA 02138 USA

[2] Harvard Univ, Sch Med, Cambridge, MA 02138 USA

[3] Harvard Univ, Dept Stem Cell & Regenerat Biol, Cambridge, MA 02138 USA

[4] Massachusetts Gen Hosp, Ctr Canc, Boston, MA 02114 USA

[5] Massachusetts Gen Hosp, Ctr Regenerat Med, Boston, MA 02114 USA

[6] Harvard Stem Cell Inst, Cambridge, MA 02138 USA

来源：

GENES & DEVELOPMENT | 2010年 / 24卷 / 20期

关键词：

ES cells; iPS cells; pluripotency; reprogramming; EMBRYONIC STEM-CELLS; PRIMORDIAL GERM-CELLS; HUMAN SOMATIC-CELLS; HUMAN IPS CELLS; NUCLEAR TRANSFER; GENE-EXPRESSION; HUMAN FIBROBLASTS; DNA METHYLATION; MOTOR-NEURONS; SELF-RENEWAL;

D O I：

10.1101/gad.1963910

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The generation of induced pluripotent stem cells (iPSCs) from somatic cells demonstrated that adult mammalian cells can be reprogrammed to a pluripotent state by the enforced expression of a few embryonic transcription factors. This discovery has raised fundamental questions about the mechanisms by which transcription factors influence the epigenetic conformation and differentiation potential of cells during reprogramming and normal development. In addition, iPSC technology has provided researchers with a unique tool to derive disease-specific stem cells for the study and possible treatment of degenerative disorders with autologous cells. In this review, we summarize the progress that has been made in the iPSC field over the last 4 years, with an emphasis on understanding the mechanisms of cellular reprogramming and its potential applications in cell therapy.

引用

页码：2239 / 2263

页数：25

共 256 条

[1] Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes [J].