Different susceptibility to oxidation of proline and arginine residues of apolipoprotein B-100 among subspecies of low density lipoproteins

被引：21

作者：

Pietzsch, J ^{[1
]}

Julius, U ^{[1
]}

机构：

[1] Tech Univ, Med Fac Carl Gustav Carus, Inst & Policlin Clin Metab Res, D-01307 Dresden, Germany

来源：

FEBS LETTERS | 2001年 / 491卷 / 1-2期

关键词：

low density lipoprotein; apolipoprotein B-100; protein oxidation; atherogenesis;

D O I：

10.1016/S0014-5793(01)02181-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

gamma -Glutamyl semialdehyde is a primary oxidation product of apolipoprotein (apo) B-100 proline (Pro) and arginine (Arg) side chain residues, By reduction gamma -glutamyl semialdehyde forms 5-hydroxy-2-aminovaleric acid (HAVA). Here me describe the application of sensitive and specific HAVA measurement to characterize the formation of gamma -glutamyl semialdehyde in several domains of apoB-100 in LDLI (S-f 7-12) and LDL2 (S-f 0-7) subfractions subjected to oxidative damage in the presence of iron in vitro. Results suggest that susceptibility of apoB-100 Pro and Arg residues toward oxygen radicals drastically changes along the lipoprotein metabolic cascade. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：123 / 126

页数：4

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