Ratiometric oxygen sensing: detection of dual-emission ratio through a single emission filter

被引：39

作者：

Kostov, Y

Harms, P

Pilato, RS

Rao, G

机构：

[1] Univ Maryland, Inst Biotechnol, Ctr Med Biotechnol, Baltimore, MD 21201 USA

[2] LUMET, Silver Spring, MD 20905 USA

来源：

ANALYST | 2000年 / 125卷 / 06期

关键词：

D O I：

10.1039/b002348p

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new method and device for the ratiometric measurement of oxygen concentration are presented. They are based on the use of a dual-emission oxygen-sensitive dye. The method allows the exclusion of the influence of emission overlap. The detection of the dual-emission ratio is performed using a single long-pass emission filter. The device described is simpler than the widely used lifetime instruments and could easily be a stand-alone low-cost instrument.

引用

收藏

页码：1175 / 1178

页数：4

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