Recovery and identification of West Nile virus from a hawk in winter

被引:90
作者
Garmendia, AE
Van Kruiningen, HJ
French, RA
Anderson, JF
Andreadis, TG
Kumar, A
West, AB
机构
[1] Univ Connecticut, Dept Pathobiol, Storrs, CT 06269 USA
[2] Connecticut Agr Expt Stn, New Haven, CT 06504 USA
[3] NYU, Med Ctr, Dept Pathol, New York, NY 10016 USA
关键词
D O I
10.1128/JCM.38.8.3110-3111.2000
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
West Nile virus was recovered from the brain of a red-tailed hawk that died in Westchester County, N.Y., in February 2000, Multiple foci of glial cells, lymphocytes, and a few pyknotic nuclei were observed in the brain. Three to 4 days after inoculation of Vero cells with brain homogenates, cytopathic changes were detected. The presence of West Nile virus antigen in fixed cells or cell lysates was revealed by fluorescent antibody testing or enzyme-linked immunosorbent assay, respectively. Furthermore, Reverse transcriptase-PCR with primers specific for the NS3 gene of West Nile virus resulted in an amplicon of the expected size (470 bp), Electron microscopy of thin sections of infected Vero cells revealed the presence of viral particles approximately 40 nm in diameter, within cytoplasmic vesicles. The demonstration of infection with the West Nile virus in the dead of the winter, long after mosquitoes ceased to be active, is significant in that it testifies to the survival of the virus in the region beyond mosquito season and suggests another route of transmission: in this case, prey to predator.
引用
收藏
页码:3110 / 3111
页数:2
相关论文
共 5 条
  • [1] Isolation of West Nile virus from mosquitoes, crows, and a Cooper's hawk in Connecticut
    Anderson, JF
    Andreadis, TG
    Vossbrinck, CR
    Tirrell, S
    Wakem, EM
    French, RA
    Garmendia, AE
    Van Kruiningen, HJ
    [J]. SCIENCE, 1999, 286 (5448) : 2331 - 2333
  • [2] Identification of a Kunjin/West Nile-like flavivirus in brains of patients with New York encephalitis
    Briese, T
    Jia, XY
    Huang, C
    Grady, LJ
    Lipkin, WI
    [J]. LANCET, 1999, 354 (9186) : 1261 - 1262
  • [3] Centers for Disease Control and Prevention (CDC), 1999, MMWR Morb Mortal Wkly Rep, V48, P845
  • [4] Origin of the West Nile virus responsible for an outbreak of encephalitis in the northeastern United States
    Lanciotti, RS
    Roehrig, JT
    Deubel, V
    Smith, J
    Parker, M
    Steele, K
    Crise, B
    Volpe, KE
    Crabtree, MB
    Scherret, JH
    Hall, RA
    MacKenzie, JS
    Cropp, CB
    Panigrahy, B
    Ostlund, E
    Schmitt, B
    Malkinson, M
    Banet, C
    Weissman, J
    Komar, N
    Savage, HM
    Stone, W
    McNamara, T
    Gubler, DJ
    [J]. SCIENCE, 1999, 286 (5448) : 2333 - 2337
  • [5] ODEROLA HA, 1977, ARCH VIROL, V54, P251