Identification of the targets of clonal deletion in an unmanipulated thymus

被引：25

作者：

Cho, HJ ^{[1
]}

Edmondson, SG ^{[1
]}

Miller, AD ^{[1
]}

Sellars, M ^{[1
]}

Alexander, ST ^{[1
]}

Somersan, S ^{[1
]}

Punt, JA ^{[1
]}

机构：

[1] Haverford Coll, Dept Biol, Haverford, PA 19041 USA

来源：

JOURNAL OF IMMUNOLOGY | 2003年 / 170卷 / 01期

关键词：

D O I：

10.4049/jimmunol.170.1.10

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Autoreactive thymocytes can be eliminated by clonal deletion during their development in the thymus. The precise developmental stage(s) at which clonal deletion occurs in a normal thymus has been difficult to assess, in large part because of the absence of a specific marker for TCR-mediated apoptosis. In this report, we reveal that Nur77 expression can be used as a specific marker of clonal deletion in an unmanipulated thymus and directly identify TCR(int)CD4(+)CD8(+) and semimature CD4(+)CD8(-) thymocytes as the principal targets of deletion. These data indicate that clonal deletion normally occurs at a relatively late stage of development, as cells mature from CD4(+) CD8(+) thymocytes to single-positive T cells.

引用

页码：10 / 13

页数：4

共 44 条

[1] Costimulatory signals are required for induction of transcription factor Nur77 during negative selection of CD4+CD8+ thymocytes [J].