Cellular response to Mineral Trioxide Aggregate

被引:262
作者
Koh, ET
McDonald, F
Ford, TRP
Torabinejad, M
机构
[1] United Med & Dent Sch Guys & St Thomas, Dept Conservat Dent, London SE1 7EH, England
[2] Loma Linda Univ, Dept Endodont, Loma Linda, CA 92350 USA
关键词
D O I
10.1016/S0099-2399(98)80074-5
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
This investigation studied the cytomorphology of osteoblasts in the presence of Mineral Trioxide Aggregate (MTA) and examined cytokine production. MTA and Intermediate Restorative Material (IRM) were prepared and placed in separate Petri dishes. Osteoblasts (cell-line MG-63), grown to confluence in Hams F12/Dulbecco's modified Eagle's medium, were seeded into the dishes, which were incubated for 1 to 7 days. The specimens were viewed by scanning electron microscopy. For cytokine evaluation, cells were grown either alone or in other dishes containing the test materials for 1 to 144 h. Media were removed for ELISA analysis of interleukin (IL)-1 alpha, IL-1 beta, IL-6, and macrophage colony-stimulating factor. Scanning electron microscopy revealed healthy cells in contact with MTA at 1 and 3 days; in contrast, cells in the presence of IRM appeared rounded. The ELISA assays revealed raised levels of all ILs at all periods when cells were grown in the presence of MTA; in contrast, cells grown alone or with IRM produced undetectable amounts. The macrophage colony-stimulating factor was produced by cells irrespective of the group. It seems that MTA offers a biologically active substrate for bone cells and stimulates IL production.
引用
收藏
页码:543 / 547
页数:5
相关论文
共 15 条
[1]   RETROGRADE FILLING MATERIALS - A RETROSPECTIVE SUCCESS-FAILURE STUDY OF AMALGAM, EBA, AND IRM [J].
DORN, SO ;
GARTNER, AH .
JOURNAL OF ENDODONTICS, 1990, 16 (08) :391-393
[2]   PLAQUE FORMATION AND ISOLATION OF PURE LINES WITH POLIOMYELITIS VIRUSES [J].
DULBECCO, R ;
VOGT, M .
JOURNAL OF EXPERIMENTAL MEDICINE, 1954, 99 (02) :167-182
[3]   CYTOKINE AND HORMONAL-STIMULATION OF HUMAN OSTEOSARCOMA INTERLEUKIN-11 PRODUCTION [J].
ELIAS, JA ;
TANG, WL ;
HOROWITZ, MC .
ENDOCRINOLOGY, 1995, 136 (02) :489-498
[4]   Using mineral trioxide aggregate - As a pulp-capping material [J].
Ford, TRP ;
Torabinejad, M ;
Abedi, HR ;
Bakland, LK ;
Kariyawasam, SP .
JOURNAL OF THE AMERICAN DENTAL ASSOCIATION, 1996, 127 (10) :1491-1494
[6]  
KETTERING JD, 1994, PATHWAYS PULP, P363
[7]  
RAPPAPORT H M, 1964, Oral Surg Oral Med Oral Pathol, V18, P785, DOI 10.1016/0030-4220(64)90480-3
[8]  
SPANGBERG LSW, 1990, EXPT ENDODONTICS, P173
[9]   OSTEOBLASTS MEDIATE INTERLEUKIN-1 STIMULATION OF BONE-RESORPTION BY RAT OSTEOCLASTS [J].
THOMSON, BM ;
SAKLATVALA, J ;
CHAMBERS, TJ .
JOURNAL OF EXPERIMENTAL MEDICINE, 1986, 164 (01) :104-112
[10]   CYTOTOXICITY OF 4 ROOT END FILLING MATERIALS [J].
TORABINEJAD, M ;
HONG, CU ;
FORD, TRP ;
KETTERING, JD .
JOURNAL OF ENDODONTICS, 1995, 21 (10) :489-492