Identification and characterization of chicken interleukin-16 cDNA

被引：33

作者：

Min, WG ^{[1
]}

Lillehoj, HS ^{[1
]}

机构：

[1] USDA ARS, Parasite Biol Epidemiol & Syst Lab, Anim & Nat Resources Inst, Beltsville Agr Res Serv, Beltsville, MD 20705 USA

来源：

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY | 2004年 / 28卷 / 02期

关键词：

chicken; interleukin-16; chemotaxis;

D O I：

10.1016/S0145-305X(03)00133-2

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Interleukin-16 is an inflammatory cytokine synthesized as a precursor protein (pro-IL-16). Based on sequence data from an EST cDNA library prepared from intestinal intraepithelial lymphocytes of Eimeria-infected chickens, we identified a cDNA that contained a full-length open reading frame of pro-IL-16. The encoded protein, predicted to consist of 607 amino acids, showed 86% sequence identity to duck pro-IL-16 and 49-52% identity to various mammalian homologues. By Northern blot analysis, IL-16 transcripts were identified in chicken lymphoid tissues but none of the non-lymphoid tissues examined. A recombinant protein containing the 149 C-terminal amino acids of pro-IL-16, expressed in COS-7 cells, showed chemoattractant activity for splenic lymphocytes. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：153 / 162

页数：10

共 41 条

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