Ion discrimination during ion accumulation in a quadrupole interface external to a Fourier transform ion cyclotron resonance mass spectrometer

被引:41
作者
Belov, ME [1 ]
Nikolaev, EN [1 ]
Harkewicz, R [1 ]
Masselon, CD [1 ]
Alving, K [1 ]
Smith, RD [1 ]
机构
[1] Pacific NW Natl Lab, Environm Mol Sci Lab, Richland, WA 99352 USA
基金
俄罗斯基础研究基金会;
关键词
electrospray; FTICR-MS (Fourier transform ion cyclotron resonance mass spectrometry); selective external ion accumulation; ion discriminations; ion stability;
D O I
10.1016/S1387-3806(01)00434-1
中图分类号
O64 [物理化学(理论化学)、化学物理学]; O56 [分子物理学、原子物理学];
学科分类号
070203 ; 070304 ; 081704 ; 1406 ;
摘要
When coupled with high-performance capillary separations, Fourier transform ion cyclotron (FTICR) mass spectrometry provides powerful new capabilities for proteomic studies. Selective ion accumulation in a two-dimensional quadrupole device external to an FTICR mass spectrometer has been shown to increase its sensitivity, dynamic range, and duty cycle. In this article, we have evaluated the operation of a linear quadrupole ion trap coupled to a 3.5-tesla FTICR mass spectrometer in the presence of high space charge. On increasing the ion population in the linear quadrupole trap, pronounced m/z discrimination was observed. We have found that the superposition of the applied quadrupole rf and dc fields, the effective dc field from space charge within the quadrupole, and the quadrupole fringing fields at the quadrupole entrance and exit result in ion instability and m/z discrimination. After optimizing experimental parameters responsible for m/z discrimination, capillary LC-separated tryptic peptide ions (from a bovine serum albumin digest) were shown to be externally accumulated and detected using the FTICR mass spectrometer without evident m/z discrimination. (C) 2001 Elsevier Science B.V.
引用
收藏
页码:205 / 225
页数:21
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