Backfat thickness in pigs is positively associated with leptin mRNA levels

被引：46

作者：

Robert, C

Palin, MF

Coulombe, N

Roberge, C

Silversides, FG

Benkel, BF

McKay, RM

Pelletier, G

机构：

[1] Agr & Agri Food Canada, Dairy & Swine Res & Dev Ctr, Lennoxville, PQ J1M 1Z3, Canada

[2] Cent Expt Farm, Ottawa, ON K1A 0C6, Canada

[3] Brandon Res Ctr Stn, Brandon, MB R7A 5Y3, Canada

[4] Univ Laval, Dept Anim Sci, Ste Foy, PQ G1K 7P4, Canada

来源：

CANADIAN JOURNAL OF ANIMAL SCIENCE | 1998年 / 78卷 / 04期

关键词：

obese gene; polymorphism; mRNA; expression; swine; leptin;

D O I：

10.4141/A98-072

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The obese (ob) gene isolated from ob/ob mice in 1994 is expressed specifically in adipose tissue. The gene product, leptin, regulates food intake, energy expenditure and puberty. Reported herein is a 2477 bp mRNA sequence for the pig obese gene, which includes the complete coding sequence. Two different ob cDNAs were identified, which differed by the inclusion or exclusion of a single glutamine codon at position +49 of the protein sequence. The pig obese gene encodes a 4.5 kb mRNA transcript, which is specific to adipose tissue. Southern blot analysis of fat and lean pigs showed a DNA polymorphism related to the lean phenotype. Differences in leptin mRNA levels showed that fat pigs have a higher level of leptin mRNA than lean pigs. These findings indicate that pig leptin expression can be associated with subcutaneous fat accumulation in pigs.

引用

收藏

页码：473 / 482

页数：10

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