Sigma A recognition sites in the Bacillus subtilis genome

被引：63

作者：

Jarmer, H

Larsen, TS

Krogh, A

Saxild, HH

Brunak, S

Knudsen, S

机构：

[1] Tech Univ Denmark, Ctr Biol Sequence Anal, DK-2800 Lyngby, Denmark

[2] Tech Univ Denmark, Sect Mol Microbiol, DK-2800 Lyngby, Denmark

来源：

MICROBIOLOGY-SGM | 2001年 / 147卷

关键词：

sigma factor; HMM; response regulator aspartate phosphatase; extended-10; region;

D O I：

10.1099/00221287-147-9-2417

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A hidden Markov model of sigma (A) RNA polymerase cofactor recognition sites in Bacillus subtilis, containing either the common or the extended -10 motifs, has been constructed based on experimentally verified sigma (A) recognition sites. This work suggests that more information exists at the initiation site of transcription in both types of promoters than previously thought. When tested on the entire B. subtilis genome, the model predicts that approximately half of the sigma (A) recognition sites are of the extended type. Some of the response-regulator aspartate phosphatases were among the predictions of promoters containing extended sites. The expression of rapA and rapB was confirmed by site-directed mutagenesis to depend on the extended -10 region.

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页码：2417 / 2424

页数：8

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