Detection of lamivudine- or adefovir-resistant hepatitis B virus mutations by a liquid array

被引：10

作者：

Liu, Hongyan ^{[1
,2
]}

Mao, Richeng ^{[1
,2
]}

Fan, Lili ^{[3
]}

Xia, Jiahui ^{[3
]}

Li, Yiliang ^{[3
]}

Yin, Yongxi ^{[3
]}

Li, Xinyan ^{[1
,2
]}

Zhao, Xu ^{[1
,2
]}

Guo, Hongying ^{[1
,2
]}

Zhu, Haoxiang ^{[1
,2
]}

Zhang, Yongmei ^{[1
,2
]}

Kang, Yaoyue ^{[1
,2
]}

Zhang, Jiming ^{[1
,2
]}

机构：

[1] Fudan Univ, Huashan Hosp, Dept Infect Dis, Shanghai 200040, Peoples R China

[2] Fudan Univ, Shanghai Med Coll, Key Lab Med Mol Virol, Shanghai 200040, Peoples R China

[3] Shanghai Fudan Zhang Jiang Biopharmaceu Corp, Shanghai 201210, Peoples R China

来源：

JOURNAL OF VIROLOGICAL METHODS | 2011年 / 175卷 / 01期

关键词：

Beads or microspheres; Chronic hepatitis B; Hepatitis B virus; Liquid array; Luminex; Lamivudine- or adefovir-resistance; LONG-TERM THERAPY; LINE PROBE ASSAY; DIPIVOXIL; PCR; MUTANTS; QUANTITATION; STRAINS;

D O I：

10.1016/j.jviromet.2011.04.005

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

A novel polymerase chain reaction (PCR)-Luminex assay was developed for rapid, accurate, and high-throughput detection of the most important hepatitis B virus (HBV) variants, including those with reverse transcriptase (RI) domain L180M, M204I/V, A181T/V/S, I233V and N2361 mutations associated with resistance to lamivudine (LAM) or adefovir (ADV). Using mixtures of mutant and wild-type HBV, this method was sufficiently sensitive for detecting 103 HBV m1(-1) and could detect minor mutants when they comprised 5% of the total viral population. Comparison of the PCR-Luminex assay with INNO-LiPA for detecting clinical LAM- or ADV-resistant chronic hepatitis B virus infection in 64 patients confirmed the following: the 2 methods were 97.9% (48 of 49) and 93.3% (14 of 15) concordant for detecting LAM- or ADV-resistance mutations, respectively. The agreement with direct sequencing was 70.3% (45 of 64). The PCR-Luminex assay or multi-analyte suspension array can detect simultaneously and efficiently minor populations HBV mutants early during infection in many clinical samples. It is a simple, cost-effective method for resistance surveillance or selecting appropriate antiviral agents and initiating timely rescue treatment before the development drug-resistance related virus or biochemical breakthrough. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：1 / 6

页数：6

共 31 条

[1]

Two sensitive PCR-based methods for detection of hepatitis B virus variants associated with reduced susceptibility to lamivudine [J].