Extra stable 2′,5′-linked RNA loops

被引：3

作者：

Hannoush, RN ^{[1
]}

Damha, MJ ^{[1
]}

机构：

[1] McGill Univ, Dept Chem, Montreal, PQ H3A 2K6, Canada

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2001年 / 20卷 / 4-7期

基金：

加拿大自然科学与工程研究理事会;

关键词：

D O I：

10.1081/NCN-100002519

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We prepared hairpins that differ in the connectivity of phosphodiester linkages in the loop (RNA vs 2', 5'-RNA). We find that the stability of the extra stable RNA hairpin 5'-rGGAC(UUCG)GUCC-3' is the same as that observed for the hairpin containing a 2',5'RNA loop, i.e. 5'-rGGAC(UUCG)GUCC-3' (where UUCG = U2'p5'U2',p5' C(2'p5')G(2'p5')). Also significant is the finding that when the stem is duplex DNA, duplex 2,5'-RNA, or DNA:2',5'-RNA, hairpins with the UUCG loop are more stable than those with UUCG loop.

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页码：1201 / 1204

页数：4

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