Fluorescence resonance energy transfer (FRET): theory and experiments

被引：41

作者：

Chirio-Lebrun, MC

Prats, M

机构：

[1] CNRS, IPBS, F-31077 Toulouse, France

[2] Univ Toulouse 3, F-31077 Toulouse, France

来源：

BIOCHEMICAL EDUCATION | 1998年 / 26卷 / 04期

关键词：

D O I：

10.1016/S0307-4412(98)80010-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fluorescence resonance energy transfer (FRET) is a powerful biophysical technique permitting macromolecular interactions between fluorescent molecules in close contact with each other to be analysed. Studies of the kinetics of association/dissociation between biological macromolecules may he carried out using this technique. Distances of interaction between donor-acceptor may be estimated by the FRET approach. (C) 1998 IUBMB. Published by Elsevier Science Ltd. All rights reserved.

引用

页码：320 / 323

页数：4

共 17 条

[1]

CANTOR CR, 1980, BIOPHYSICAL CHEM, V2

[2] FLUORESCENCE ENERGY-TRANSFER ANALYSIS OF CALMODULIN.PEPTIDE COMPLEXES [J].